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Member Information
Frederic Bushman, Ph.D.
Professor, Department of Microbiology
Email: Bushman@mail.med.upenn.edu
Website(s): http://microb230.med.upenn.edu/Development/index.html
Education: Ph. D. 1989, Harvard University
Keywords: HIV, integration, replication, mechanism, genomics
Research and/or Clinical Interests:
Research in the Bushman laboratory focuses on mechanisms of replication by retroviruses and poxviruses.
Summary:
Research in the laboratory of Dr. Frederic Bushman focuses on understanding the transfer of genetic information between cells and organisms. Phenomena as diverse as viral replication, microbial pathogenesis, immune system development and genomic evolution are all mediated in large measure by reactions that transfer and rearrange DNA sequences. Research is further focused on DNA modifying enzymes encoded by medically relevant pathogens, since these are a particularly interesting set and also important as drug targets. Major projects in the laboratory focus on two viral model systems: HIV and poxviruses.
Representative Publications:
R. Craigie, T. Fujiwara and F.D. Bushman. (1990) The in protein in Moloney murine leukemia virus processes the viral DNA ends and accomplishes their integration in vitro. Cell 62: 829-837.
F.D. Bushman, T. Fujiwara and R. Craigie. (1990) Retroviral DNA integration directed by HIV IN protein in vitro. Science 249: 1555-1558.
S. L. Butler, M. S. T. Hansen, and F. D. Bushman. (2001) A Quantitative Assay for HIV cDNA Integration In Vivo. Nature Med. 7, 631-634.
A. R. W. Schroder, P. Shinn, H. Chen, C. Berry, J. R. Ecker, and F. D. Bushman. (2002) HIV-1 Integration in the Human Genome Favors Active Genes and Local Hotspots. Cell 110, 521-529 (see also cover).
R. Mitchell, B. Beitzel, A. R. W. Schroder, P. Shinn, H. Chen, C. Berry, J. R. Ecker, and F. D. Bushman. (2004) Retroviral DNA Integration: ASLV, HIV, and MLV Show Distinct Target Site Preferences. PLoS Biology 2, E234 (see also cover).
A. Ciuffi, M. Llano, E. Poeschla, H. Marshall, C. Hoffman, J. Leipzig, P. Shinn, J. Ecker, F. Bushman. (2005) A role for LEDGF/p75 in targeting HIV DNA Integration. Nat. Med. 11, 1287-1289.
T. Diamond and F. Bushman. (2005) Division of labor within HIV-1 integrase complexes: determinants of catalysis and target DNA capture. J. Virol. 79, 15376-15387.
A. Ciuffi, R. S. Mitchell, C. Hoffmann, J. Leipzig, P. Shinn, J. R. Ecker, F. D. Bushman. (2006) Integration site selection by HIV-based vectors in dividing and growth-arrested IMR-90 lung fibroblasts. Mol. Therapy 13, p. 366-373.
K. Yoder, A. Sarasin, K. Kraemer, M. McIhatton, F. D. Bushman, R. Fishel. The DNA Repair Genes XPB and XPD Defend Cells from Retroviral Infection. Proc. Natl. Acad. Sci. USA, 2006, EPub.
