 |
 |
 |
 |
 |
 |
 |
Member Information
Carl June, MD
Professor, Department of Pathology and Laboratory Medicine
Office Phone: 215-573-5745
Office Fax: 215-573-8590
Email: cjune@mail.med.upenn.edu
Website(s): http://www.uphs.upenn.edu/abramson/june.html
Education: MD 1979, Baylor College of Medicine
Keywords: Immunotherapy, lymphocyte activation
Research and/or Clinical Interests:
Immunotherapy of HIV infection
Summary:
There are two themes in the laboratory. One is to develop and test novel forms of immunotherapy for cancer and chronic infections. The other project involves determining mechanisms of lymphocyte activation.
Over the last five years my laboratory has been studying the potential use of adoptive immunotherapy for HIV infection. We have developed a large-scale tissue culture technique that permits the efficient propagation of polyclonal HIV CD4 and CD8 T cells from patients with HIV infection. Several clinical trials involving adoptive immunotherapy of autologous and allogeneic T cells are in process. Other trials involve infusions of gene modified T cells.
Another project in my laboratory involves determining mechanisms of T cell signal transduction. For the past ten years these studies have been focused on CD28 and CTLA-4. More recently we have also been studying mechanisms that regulate chemokine receptor expression and signal transduction in T cells. In addition, we have studied the role of telomerase expression, telomere maintenance and replicative senescence in T cells.
Representative Publications:
Linette,G.P., Hartzman,R.J., Ledbetter,J.A., and June,C.H. 1988. HIV-1-infected T cells show a selective signaling defect after perturbation of CD3/antigen receptor. Science 241:573-576.
Levine,B.L., Mosca,J., Riley,J.L., Carroll,R.G., Vahey,M.T., Jagodizinski,L., Wagner,K.F., Mayers,D.L., Burke,D.S., Weislow,O.S. and June C.H.. 1996. Antiviral Effect and Ex Vivo CD4+ T Cell Proliferation In HIV-Positive Patients as a Result of CD28 Costimulation. Science 272:1939-1943.
Levine,B.L., Mosca,J., Riley,J.L., Carroll,R.G., Vahey,M.T., Jagodizinski,L., Wagner,K.F., Mayers,D.L., Burke,D.S., Weislow,O.S. et al. 1996. Antiviral Effect and Ex Vivo CD4+ T Cell Proliferation In HIV-Positive Patients as a Result of CD28 Costimulation. Science 272:1939-1943.
Riley,J.L., Schlienger,K., Blair PB, Carreno,B.M., Craighead,N., Kim,D., Carroll,R.G., and June C.H. 2000. Modulation of Susceptibility to HIV-1Infection by the Cytotoxic T Lymphocyte Antigen 4 Costimulatory Molecule. J Exp Medicine 191:1987-1998.
Levine,B.L., Bernstein WB, Aronson NE, Schlienger K, Cotte J, Perfetto S, Humphries MJ, Ratto-Kim S, Birx DL, Steffans C and June CH . 2002. Adoptive Transfer of Costimulated CD4 + T cells Induces Expansion of Peripheral T Cells and Decreased CCR5 Expression in HIV Infection. Nature. Med. 8:47-53.
Riley, J. L., B. L. Levine, N. Craighead, T. Francomano, D. Kim, R. G. Carroll, and C. H. June. Naive and memory CD4 T cells differ in their susceptibility to HIV-1 infection following CD28 costimulation: Implications for transmission and pathogenesis. J Virol. 72:8273-8280, 1998.
Ward, S. G., and C. H. June. T lymphocyte activation. In: Encyclopedia of Immunology, edited by I. M. Roitt and P. J. Delves, London : Academic Press, 1999, p. 2323-2329.
Levine, B. L., J. Mosca, J. L. Riley, R. G. Carroll, M. T. Vahey, L. Jagodizinski, K. F. Wagner, D. L. Mayers, D. S. Burke, O. S. Weislow, D. C. St.Louis, and C. H. June. Antiviral Effect and Ex Vivo CD4+ T Cell Proliferation In HIV-Positive Patients as a Result of CD28 Costimulation. Science 272:1939-1943, 1996.
Blair, P. J., J. L. Riley, B. L. Levine, K. P. Lee, N. Craighead, T. Francomano, S. J. Perfetto, G. S. Gray, B. M. Carreno, and C. H. June. CTLA-4 Ligation Delivers a Unique Signal to Resting CD4 T Cells that Promotes Cell Survival but not IL-2 Secretion. J Immunol 160:12-15, 1998. 1997.
Kaushal, S., A. L. Landay, M. Lederman, E. Connick, J. Spritzler, D. R. Kuritzkes, H. Kessler, B. L. Levine, D. St.Louis, and C. H. June. Increases in T-cell telomere length in HIV infection after antiretroviral combination therapy for HIV-1 infection implicate distinct population dynamics in CD4+ and CD8+ T-cells. Clinical Immunology 92:14-24, 1999.
