 |
 |
 |
 |
 |
 |
 |
Member Information
Hao Shen, PhD
Associate Professor of Microbiology
Office Phone:
Office Fax:
Email:
Website(s): http://www.med.upenn.edu/micro/faculty/shen.html
Education:
Keywords: T cell memory and Vaccines
Research and/or Clinical Interests:
Understanding the factors that influence the generation and maintenance of functional T cell memory
Summary:
We are interested in understanding how the microbial virulence strategies affect the nature and magnitude of the host response and how the resulting immune response influences the course of infection. We employ a multidisciplinary approach that combines recent technical and conceptual advances in molecular genetics, microbial pathogenesis, cell biology and immunology. By genetic manipulation of the microbes, we are identifying different aspects of microbial antigens that influence their ability to induce a T cell response and to serve as a protective target. We are also combining our ability to genetically manipulate the microbes with the use of knock-out mice to examine how various immune effectors counter different microbial virulence factors and to identify new immune correlates of protection. The long-term objective of these basic studies is to provide a platform for rational design of effective vaccines to combat today's complex and evolving diseases such as HIV.
Representative Publications:
Shedlock DJ, Shen H. Requirement for CD4 T cell help in generating functional CD8 T cell memory. Science 300:337-9, 2003
Shedlock DJ, Whitmire JK, Tan J, MacDonald AS, Ahmed R, Shen H. Role of CD4 T cell help and costimulation in CD8 T cell responses during Listeria monocytogenes infection. J Immunol 170:2053-63, 2003
Shen H, Whitmire JK, Fan X, Shedlock DJ, Kaech SM, Ahmed R. A specific role for B cells in the generation of CD8 T cell memory by recombinant Listeria monocytogenes. J Immunol. 170:1443-51, 2003.
Troy AE, Shen H. Cutting edge: homeostatic proliferation of peripheral T lymphocytes is regulated by clonal competition. J Immunol. 170:672-6, 2003.
Zenewicz LA, Foulds KE, Jiang J, Fan X, Shen H. Nonsecreted bacterial proteins induce recall CD8 T cell responses but do not serve as protective antigens. J Immunol. 169:5805-12, 2002.
San Mateo LR, Chua MM, Weiss SR, Shen H. Perforin-mediated CTL cytolysis counteracts direct cell-cell spread of Listeria monocytogenes. J Immunol. 169:5202-8, 2002.
Foulds KE, Zenewicz LA, Shedlock DJ, Jiang J, Troy AE, Shen H. Cutting edge: CD4 and CD8 T cells are intrinsically different in their proliferative responses. J Immunol. 168(4):1528-32, 2002.
Ochsenbein AF, Karrer U, Klenerman P, Althage A, Ciurea A, Shen H, Miller JF, Whitton JL, Hengartner H, Zinkernagel RM. A comparison of T cell memory against the same antigen induced by virus versus intracellular bacteria. Proc Natl Acad Sci U S A. 96:9293-8,1999.
Shen H, Miller JF, Fan X, Kolwyck D, Ahmed R, Harty JT. Compartmentalization of bacterial antigens: differential effects on priming of CD8 T cells and protective immunity. Cell. 92:535-45, 1998.
Shen H, Slifka MK, Matloubian M, Jensen ER, Ahmed R, Miller JF. Recombinant Listeria monocytogenes as a live vaccine vehicle for the induction of protective anti-viral cell-mediated immunity. Proc Natl Acad Sci U S A 92:3987-91, 1995.
