How to do Gram Stains


List of Reagents and Instruments

Equipment

Bunsen burner, alcohol-cleaned microscope slide, water

Reagents

Crystal violet, Gram's iodine solution, acetone/ethanol (50:50 v:v), 0.1% basic fuchsin solution

Procedures

1. Prepare a Slide Smear:

2. Gram Staining:

3. Quality control:

3. Examine the finished slide under a microscope.


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