|
Home>Faculty
List>Dr. Payne
|
Aimee S. Payne, M.D., Ph.D. |
||
Education Pemphigus is a model organ-specific autoimmune disease in which antibodies against desmosomal cell adhesion molecules known as desmogleins cause potentially fatal skin blistering. We are interested in defining the autoimmune repertoire in pemphigus at the molecular genetic level in order to better understand disease pathogenesis and develop more specific, and potentially safer, treatments. Using phage display, we have isolated monoclonal anti-desmoglein antibodies from patients with active pemphigus vulgaris (PV). Genetic analysis shows that a limited number of antibody genes encode the human PV autoantibody repertoire, with different genes for pathogenic and non-pathogenic antibodies, and shared genes even among different PV patients. Targeting of the heavy chain variable region genes identified in the first PV patient library blocked the pathogenic activity of the patient's serum against cultured human keratinocytes, indicating the feasibility of pathogenic antibody-targeted therapy. Using the PV monoclonal antibodies that we have identified, we have shown that pathogenic anti-desmoglein antibodies prevent desmosome assembly in human keratinocytes. Current studies are focused on better defining mechanisms for the loss of cell adhesion. Eun Jung Choi, M.S. - Research Assistant Xuming Mao, M.D., Ph.D. - Postdoctoral Fellow Arielle Nagler - Medical Student Preety Sharma, Ph.D. - Postdoctoral Fellow Payne AS, Hanakawa Y, Amagai M, and Stanley JR. (2004) Desmosomes and disease: pemphigus and bullous impetigo. Payne AS, Ishii K, Kacir S, Lin C, Li H, Hanakawa Y, Tsunoda K, Payne AS, Yan AC, Ilyas E, Li W, Seykora JT, Young TL, Pawel BR,
Honig PJ, Camacho J, Imaizumi S, Heymann WR, and Schnur RE. (2005)
Two novel TP63 mutations associated with the ankyloblepharon,
ectodermal defects, and cleft lip and palate syndrome: a skin fragility
phenotype. Arch. Dermatol., 141, 1567-1573.
Payne AS, James WD, and Weiss RB. (2006) Dermatologic toxicity of
chemotherapeutic agents. Semin. Oncol., 33, 86-97.
Payne AS, Siegel DL, and Stanley JR. (2007) Targeting pemphigus
autoantibodies through their heavy chain variable region genes. Sharma P, Mao X, and Payne AS. (2007) Beyond steric hindrance: the
role of adhesion signaling pathways in the pathogenesis of pemphigus. Mao X and Payne AS. (2008) Seeking approval: Present and future
therapies for pemphigus vulgaris. Murrell DF, Dick S, Ahmed AR, et al. (2008) Consensus statement on Mao X, Choi EJ, Payne AS. (2009) Disruption of desmosome assembly Sharma PM, Choi EJ, Ishii K, Payne AS. (2009) Pathogenic anti- Rosenbach M, Murrell DF, Bystryn J-C, Dulay S, Dick S, Fakharzadeh Cell and Molecular Biology Graduate Group: International Pemphigus Foundation:
1993 B.S. Stanford University
2001 M.D./Ph.D. Washington University School of Medicine
2001-02 Internship Internal Medicine, Pennsylvania Hospital
2002-05 Residency Dermatology, University of Pennsylvania
Clinical Specialties
General dermatology; autoimmune blistering skin diseases
Research Areas of Interest:
Pemphigus, cell adhesion, autoimmunity
Research Summary:
Lab Members:
Alumni:
Selected
Publications
Curr. Opin. Cell Biol., 16, 536-543.
Amagai M, Stanley JR, and Siegel DL. (2005) Genetic and functional
characterization of human pemphigus vulgaris monoclonal
autoantibodies isolated by phage display.
J. Clin. Invest., 115, 888-899.
J.Invest.Dermatol., 127, 1681-1691. ![]()
J. Dermatol. Sci., 48: 1-14.
![]()
Curr. Opin. Invest. Drugs, 9(5):497-504![]()
definitions of disease, end points, and therapeutic response for
pemphigus.
J. Am. Acad. Dermatol, 58: 1043-1046![]()
by monovalent human pemphigus vulgaris monoclonal antibodies.
J.Invest.Dermatol., doi: 10.1038/jid.2008.339, in press.
desmoglein monoclonal antibodies demonstrate variable ELISA activity
due to preferential binding of mature versus proprotein isoforms of
desmoglein 3. J.Invest.Dermatol., doi: 10.1038/jid.2009.41, in
press.
SS, Hall R, Korman NJ, Lin J, Okawa J, Pandya AG, Payne AS, Rose M,
Rubenstein D, Woodley D, Vittorio C, Werth BB, Williams EA, Taylor L,
Troxel A, Werth VP. (2009) Reliability and convergent validity of two
outcome instruments for pemphigus.
J.Invest.Dermatol, doi: 10.1038/jid.2009.72., in press.
http://www.med.upenn.edu/camb/faculty/cbp/payne.html
http://www.pemphigus.org/
|
Privacy Statement | Legal
Disclaimer
The University of Pennsylvania Health System. © 2005. The Trustees of University of Pennsylvania |