Nicholas A. Kefalides, M.D., Ph.D.
Dr. Kefalides’ laboratory is involved with studies on the cell and molecular biology of extracellular matrix. Studies include the structure and function of basement membrane components, such as type IV collagen and laminin, and their role in cell adhesion and tumor metastasis. Recent studies focus on the role of type IV collagen in the regulation of the inflammatory response at the capillary level and specifically the role of the non-collagenous domain of the a3 chain of type IV collagen in down-regulating the activation of PMN’s. The same domain of the a3 chain was shown to inhibit tumor cell replication and angiogenesis in vivo and in vitro. The type IV collagen peptides bind to a receptor complex CD47- avb3 to inititate the inhibitory activity.
Shahan TA, Ziaie Z, Fawzi A, Pasco S, Bellon G, Monboisse J-C, Kefalides NA: Identification of CD47/integrin associated protein and avb3 as two receptors for the alpha3 (IV) chain of type IV collagen on tumor cells. Cancer Research. 59: 4584-4590, 1999.
Kefalides NA and Ziaie Z: Herpes Simplex Virus infection of vascular cells and its effects on extracellular matrix. In “Viruses and Atherosclerosis,” (Edited by D.P. Hajjar and S.M. Schwartz). Harwood Academic Publishers, England, pp. 107-127, 1999.
Shahan TA, Fawzi A, Bellon G, Monboisse J-C, Kefalides NA. Regulation of Tumor Cell Chemotaxis by Type IV Collagen is mediated by a Ca2+-Dependent Mechanism Requiring CD47 and the Integrin alpha(V)beta(3). J. Biol. Chem 275:4796-4802, 2000.