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Rubin, M.D., Ph.D.
The Rubin laboratory is involved in several projects. 1. Pathogenesis of dormancy in Mycobacterium tuberculosis: It is widely and believed that oxygen limitation, amino acid starvation and carbon source restriction are involved in establishing and maintaining Mycobacterium tuberculosis in a dormant state. Correspondingly, emergence from dormancy is related to a partial or complete amelioration of these conditions. The lab identified three genetic and enzyme systems that comprise regulatory networks in M.TB that may be involved in pathogenesis. 2. Enzymology and cell biology of serine proteases and serine protease inhibitors: Serine proteases and serine protease inhibitors (serpins) play critical roles in inflammation, coagulation, growth and development. The lab has proposed a general model for the mechanism of inhibition of serine proteases by serine protease inhibitors based on site directed mutagenesis, atomic resolution crystal structures and NMR spectroscopic analyses, and is exploring the consequences and extensions of this model. 3. Biomolecular Computation: A new area of investigation is known as biomolecular computation where complex computational operations are carried out using biomolecules, in particular using DNA. The lab showed how macromolecules can be manipulated to carry out fundamental logical operations and can be wired together as reversible logic gates. The lab is collaborating with members of the School of Engineering on modeling complex biological behavior using a hybrid systems approach that combines continuous and stochastic modalities.
Avarbock, D., Avarbock,
A. and Rubin, H. Differential Regulation of Opposing Activities by the
Amino-Acylation State of a tRNA-Ribosome-mRNA-RelMtb Complex. Biochemistry
39, 11640-11648, 2000.
Estbanez-Perpina, E., Fuentes-Prior, P., Belorgey, D., Braun, M., Kiefersauer, K., Maskos, K., Huber, R., Rubin, H. and Bode, W. Crystal Structure of the Caspase Activator Human Granzyme B, a Proteinase Highly Specific for an Asp-P1 Residue. Biological Chemistry 381, 1203–1214, 2000.
Kana BD., Weinstein
EA., Avarbock D., Dawes SS., Rubin H., Mizrahi V. Characterization of
the cydAB-Encoded Cytochrome bd Oxidase from Mycobacterium smegmatis.
J. Bacteriol. 183,7076-7086, 2001