Resident Physician, PGY-5

Department of Neurosurgery, Silverstein 3
The Hospital of the University of Pennsylvania
3400 Spruce Street
Philadelphia, PA 19104
(215) 662-3487
melandee.brown@uphs.upenn.edu

Biographical Sketch

Education

• Columbia University College of Physicians and Surgeons, New York, NY Doctor of Medicine, May 2002
• Brown University, Providence, RI, Bachelor of Science in Neural Sciences, May 1995

Postgraduate Training

• Hospital of the University of Pennsylvania, Philadelphia, PA, Pre-Residency Fellowship, Department of Neurological Surgery April 2005 - Present
• Columbia University, New York, NY, Resident, Department of Neurological Surgery, July 2003 – November 2004
• Columbia University, New York, NY, Intern, Department of Surgery, July 2002 - June 2003

Awards, Honors, and Membership in Honorary Societies

• Black and Latino Student Organization. Certificate of Achievement. Columbia University, 2002.
• Becker Fellowship. Scholarship award for research in brain tumors. Columbia University, 2000-2001.
• SIGN Travel Award for Neurology. Columbia University, 2000.
• Ford Scholarship. Academic Scholarship for a degree in Medicine. Columbia University, 1997-2001.
• Brown Community Outreach Award for Outstanding Student Contribution to Public Service, 1995.
• NIH Fellowship for Student Research, Brown University 1993.

Research Experience

• Bartoli Brain Tumor Laboratory, Columbia University New York, NY July 2000 - June 2001 and July 2003 - May 2004.
• Investigated the efficacy of anti-angiogenic and anti-edema compounds in vitro and in vivo using an intracranial syngeneic rat glioma model. Developed an in vivo model to demonstrate changes in brain tumor blood vessels following anti-angiogenic and anti-edema therapy using florescent angiography and digital imaging. Defined the angiogenic profiles of ex vivo glioblastoma multiforme specimens through the use of ELISA and functional assays. Investigated the effects of in vitro culture on the secretion of immunomodulatory cytokines by glioblastomas. Used MRI imaging to investigate the effects of corticosteroids and novel anti-edema compounds on rat brain tumor vascular permeability.
• Research Associate Neurology, Children's Hospital Boston, MA August 1996 - August 1997.
• Demonstrated the effects of phosphocreatine and guanidine analogues on energy storage buffers in brain mitochondria. Conducted MRI studies on the effects of creatine analogues on resistance to hypoxia injury in neonates.
•  Brown University Medical Research Laboratories, Providence, RI September 1994 - June 1995.
• Investigated the role of Apolipoprotein E and beta-amyloid in the pathology of Alzheimer's disease. Developed a protocol for neuronal tissue culture.
• Research Associate Clinical Immunology, Roger William's Hospital Clinical Immunology and Pathology, Providence, RI Summer 1994.
• Documented the effects of pre-clinical pharmaceuticals on the modulation of macrophage cytokine production. Developed a protocol for drug delivery and macrophage harvest. Performed cell counts and cell line maintenance.
• NIH Research Internship Neurological Diseases and Movement Disorders NIH/NINDS Bethesda, Maryland Summer 1993.
• Conducted research on cortical and subcortical functional abnormalities in patients with Alzheimer's disease, Parkinson's disease and schizophrenia through the use of PET imaging.

Articles

Brown MD, Mandigo CE, Anderson RC, Parsa AT, Bruce JN. Local delivery of Endostatin does not improve animal survival in an intracranial 9L glioma model. (In preparation)

Anderson RC, Elder JB, Brown MD, Mandigo CE, Anderson DE, Parsa AT, Senatus PB, Bruce JN. Ex vivo analysis of human glioblastomas indicates immunologic heterogeneity and lack of functional co-stimulation. Journal of Clinical Investigation. (Accepted Oct 2004)

Senatus PB, Li Y, Mandigo CE, Nichols G, Mao Y, Moise G, Brown MD, Anderson RC, Fine R, Bruce JN. Restoration of p53 function for selective fas mediated apoptosis for human and rat glioma cells by a COOH-terminal peptide. Molecular Cancer Ther. 2006. Jan; 5 (1): 20-8

Anderson RC, Elder JB, Brown M, Mandigo CE, Parsa AT, Bruce JN. Changes in Immunologic phenotype of human malignant glioma cells after Passaging In Vitro. Clinical Immunology. 2002. 102(1):84-95.

Holtzman D, Brown M. Brain ATP Metabolism in Hypoxia Resistant Mice Fed Guanidinoproprionic Acid. Developmental Neuroscience, 1998; 20(4-5): 469-77.

Abstract

Brown MD, Canoll P, Mandigo CE, Syed O, Senatus PB, Bruce JN. Primary Glioblastoma Cell Lines Express Corticotropin-Releasing Factor Receptors 1 and 2. Congress of Neurological Surgeons, Annual Meeting, October 2004.

Brown MD, Mandigo CE, Weicker M, Parsa AT, Bruce JN. Local Delivery of Endostatin Does Not Improve Animal Survival in an Intracranial Glioma Model. American Association of Neurological Surgeons, Annual Meeting, May 2004.

Anderson RC, Brown MD, Elder JB, Mandigo CE, Parsa AT, Anderson DE, Bruce JN. Release of immunosuppressive cytokines from glioblastomas ex vivo. American Association of Neurological Surgeons, Annual Meeting, April 2002.

Anderson RC, Brown MD, Elder JB, Mandigo CE, Parsa AT, Anderson DE, Bruce JN. Analysis of immunosuppressive cytokines released by glioblastomas ex vivo. American Association of Neurological Surgeons, Tumor Section, April 2002.

Anderson RC, Elder JB, Brown MD, Mandigo CE, Parsa AT, Senatus PB, Anderson DE, Bruce JN. Exogenous B7 Co-stimulation Overcomes Inherent Immune Evasion in Ex vivo Human Glioblastomas. Congress of Neurological Surgeons, Annual Meeting, 2002.

Brown MD, Anderson RC, Mandigo CE, Parsa AT, Bruce JN. Secretion of Angiogenic Factors by Glioblastomas and Normal Brain Ex Vivo. Congress of Neurological Surgeons, Annual Meeting, September 2001.

Brown MD, Anderson RC, Mandigo CE, Parsa AT, Bruce JN. Secretion of Immunomodulatory Cytokines by Human Glioblastoma Specimens Ex Vivo. Congress of Neurological Surgeons. Annual Meeting, September 2001.

Anderson RC, Elder JB, Brown MD, Anderson D, Chi J, Parsa AT, Bruce JN. Changes in the Expression of Immunologic Cell Surface Molecules on Human Glioma Cells after Multiple Passages In Vitro: Implications for Pre-Clinical Evaluation of Immunotherapy. American Association of Neurological Surgeons, Annual Meeting, April 2001.

Senatus P, Mandigo CE, Anderson RC, Brown MD. Toxicity of Novel Chimeric p53 Peptide to Rat and Human Malignant Gliomas In vitro and In vivo. American Association on Neurological Surgeons, Annual Meeting, April 2001.

Anderson RC, Elder JB, Mandigo CE, Brown MD, Parsa AT, Anderson D, Bruce JN. Ex vivo Analysis of Human Glioblastomas Demonstrates Immunological Heterogeneity and Functional Lack of Co-Stimulation: Implications for CNS Tumor Evasion. J Neuro. Onc., May 2001.

Chapters

Bruce JN, Parsa AT, Brown MD, Anderson RC, McCormick P, Feldstein N. Ependymoma. In online textbook, E-Medicine. 2001.

Bruce JN, Senatus P, Elder JB, Brown MD, Parsa AT. Astrocytoma. In online textbook, E-Medicine 2001.

Oral Glioblastoma Cell Lines Express Corticotropin-Releasing Factor Receptors 1 and 2. Grand Rounds Presentations Lecture, Department of Neurological Surgery, Columbia University, New York, October 2004.

Local Delivery of Endostatin Does Not Improve Animal Survival in an Intracranial Glioma Model. Grand Rounds Lecture, Department of Neurological Surgery, Columbia University, New York, May 2004.

Meningitis in the Immunocompromised Host. Department of Neuropathology. Columbia University College of Physicians and Surgeons, New York, September 2001.

The Role of Secreted Angiogenic Factors in Vascular Proliferation in Glioblastomas. Dean's Day, Columbia University College of Physicians and Surgeons, New York, May 2001.

Secretion of Angiogenic Factors by Ex vivo Human Glioblastomas and Normal Brain. Eastern Student Research Forum, University of Miami, Miami, Florida, February 2001