Development of a nervous tissue construct for
Top: Neurons are plated on two membranes and a
neural network (black) is formed.
Bottom panels: Movement of a block attached to
one membrane (yellow) via a computer controlled microstepper motor
system divides the culture and progressively separates the halves.
As the microstepper motor moves the top membrane, the portion of
the axonal bundles crossing between the top and bottom membranes
is extended inducing stretch growth of the axon bundles. These cultures
are then embedded in collagen and rolled into a tube that facilitates
removal from the device. This construct is then transplanted to
bridge spinal cord lesions.
Image Credit: Douglas H. Smith, MD, University of Pennsylvania
School of Medicine