Top: Schematic of
nervous tissue construct (green) transplanted into a spinal cord
lesion spanning three vertebrae (white).
Bottom left, upper & lower: Double and triple
labeling identified surviving dorsal root ganglia clusters with
elongated axons at the edge of transected spinal cord injury lesions
at 2 months post-transplant. Scale bars = 50µm.
Bottom middle, upper & lower: Double labeling
identified surviving elongated axon fascicles in the middle of transplanted
region at 2 months post-transplant. Scale bars = 50µm.
Bottom right, upper: Double labeling identifies
axonal outgrowth of graft axons into host tissue at the host-graft
interface, penetrating through the collagen hydrogel.
Bottom right, lower: Axonal outgrowth from nerve
graft into the host, penetrating collagen hydrogel. Scale bars =
Image Credit: Douglas H. Smith, MD, University of Pennsylvania
School of Medicine