Small Animal Imaging Facility - Services
The MR Sub-Core of the SAIF provides the instrumentation and expertise necessary to perform a broad spectrum of magnetic resonance imaging and spectroscopy studies on a wide range of biological samples including small animals (cats, rabbits, rats, mice), tissue specimens, cultured cells and tissue extracts.
This facility includes a conveniently located, well equipped surgery room used for preparing the animals for MR exams and a wide assortment of supporting equipment, i.e. anesthesia machines, MR compatible vital signs monitors (SA Instruments), infusion pumps (Harvard), heating pads, etc. A variety of perishable supplies used in animal preparation are provided by the facility.
Instrumentation
- 4.7 T 40 cm horizontal bore MR Spectrometer: (Small Animal Imaging Lab Basement of the Founders building) equipped with a 30 cm ID 3 gauss/cm and a 12 cm ID 25 gauss/cm gradient tube and interfaced to a Varian DirectDrive console. The console is configured with two broad band channels as well as a 4 channel receiver array. A variety of volume and surface coils suitable for imaging of rodents are also available.
- 9.4 T 8.9 cm vertical bore MR Specrometer: (Small Animal Imaging Lab Basement of the Founders building) equipped with a 55 mm ID 100 gauss/cm gradient tubes and interfaced to a Varian DirectDrive console. The console is configured with full imaging capabilities, three transmit/receive channels (one high band and two low band), variable temperature control, sample insert and eject capabilities, and liquids and solids probes suitable for generation of high-resolution spectra. Numerous slotted tube resonators suitable for imaging and spectroscopy of subcutaneous tumors and tissue specimens are also available.
- 9.4 T 31 cm horizontal bore MR Spectrometer: (B100 John Morgan Building) equipped with a 21 cm ID gauss/cm and a 12 cm ID gauss/cm gradient tube and interfaced to a Varian DirectDrive console.
- 11.T 55 mm vertical bore high resolution MR Spectrometer: (B96 John Morgan Building) equipped with a wide array of multi-nuclear high resolution probes and interfaced to a Bruker AMX console.
Access
Users interested in utilizing the SAIF MR resources are encouraged to contact the director of the facility, S. Pickup see below for contact information) in order to discuss protocol design before completing the SAIF research application. The prospective user then completes and submits a SAIF Research Applications form which is reviewed by the relevant core directors. Upon receiving approval of the application, the investigator must contact a member of the staff of the MR facility in order to create user accounts on the target platform and scheduling calendar. Users with sufficient experience may schedule studies, prepare animals and perform scans with minimal support from the staff. Less experienced users may opt to have the scans performed by facility staff on a fee for service basis.
All users are expected to clean up after themselves! The lab should be at least as clean when you leave as it was when you arrived. Everything in the lab should be wiped clean and returned to it's proper storage location. All trash should be placed in the proper receptacle. All tape should be removed from facility equipment and disposed of. The taped surface should also be wiped clean of any residue from the tape. All Drug Enforcement Agency controlled substances must be locked in the boxes provided when not in use.
Scheduling Policy
The policy as described below was developed in order to accommodate as many research projects as possible. The facility will not police the schedule, however, users are encouraged to notify the facility staff if a user is in violation of the policy.
- When reserving magnet time, the title should include the telephone number of the primary user so that they may be contacted if a scheduling conflict should occur. Failure to adhere to this policy may result in removal of the scheduled block from the calendar.
- No user should sign up for more than 4 hours of prime time per day prior to 72 hours before the scheduled time unless special arrangements are made with the facility director (NOTE: The need to do multiple animals is not sufficient justification of scheduling large blocks of prime time).
- Users desiring a prime time slot should sign up for one of three slots; 8:00 am to 12:00 pm, 12:00 pm to 2:00 pm and 2:00 pm to 6:00 pm. Shorter blocks of time may be scheduled but they should not span the 12:00 pm transition.
- ProDev users should not schedule any prime time earlier than 72 hours before to the desired slot.
- None of the above rules apply if a slot has not been reserved 72 hrs prior to the scheduled time.
- None of the above applies to non-prime time.
- Failure to adhere to the above policy is grounds for removal of the offending time slot from the schedule.
Online Schedule
Click Online Schedule for the calendar.
MRI and MRS Rates
Users are billed for the time that they are logged into the console at the following rates. The login times are automatically recorded by the operating system. Billing statements are generated shortly after the close of the billing cycle (first of the month) and are emailed to the PI of the study. No statement is generated for accounts that did not have any activity during a given billing cycle. The facility employs a two tiered billing schedule as described below.
- $70/hr - Mon-Fri: 9am to 5pm
- $20/hr max, $80/session - Holidays, Sat, Sun, & Mon-Fri: 5pm to 9pm
Contact Information
Founders Basement Lab: 215-662-6678
B100 John Morgan: 215-573-2534
B96 John Morgan: 215-898-6396
Stephen Pickup
SAIF Technical Director & MR Sub Core Director
Phone: 215-349-5297
Email: pickup@mail.med.upenn.edu
Weixia Liu, PhD
MR Lab Manager
Phone: 215-573-2534
Email: weixia@mail.med.upenn.edu
Anthony Mancuso
Director, Laboratory for NMR Spectroscopy of Cellular Metabolism
Phone: 215-764-5527
Email: mancusoa@uphs.upenn.edu
