|
Chacko,
S. (1973) DNA synthesis, mitosis, and differentiation in
cardiac myogenesis. Dev. Biol. 35:272774-84.
Blose, S.H., Shelanski, M.L. and Chacko,
S. (1976) Localization of bovine neurofilament antibody
to intermediate filaments in endothelial cells. J. Cell. Biol.,
70:459-466.
Chacko S., Conti,
MA, and Adelstein, RS. (1977). The effect of phosphorylation of
smooth muscle myosin on actin activation and Ca2+ regulation. Proc.
Natl. Acad. Sci. (USA). 74:129-133.
Chacko, S. (1981)
Effect of phosphorylation Ca2+ and tropomyosin on actin-activated
ATPase activity of mammalian smooth muscle myosin. Biochemistry.
20:702-703.
Chacko, S. and Rosenfeld,
A. (1982) Regulation of actin-activated hydrolysis by arterial myosin.
Proc. Natl. Acad. Sci. (USA) 79:292-296.
Miyata, H. and Chacko, S.
(1986) Role of tropomyosin in smooth muscle contraction: The effect
of tropomyosin binding to actin on actin-activation of myosin ATPase.
Biochemistry. 25:2725-2729.
Horiuchi, KY., and Chacko,
S. (1989) Caldesmon Inhibits the cooperative turning-on
of the smooth muscle heavy meromyosin by tropomyosin-actin. Biochemistry,28:9111-911.
Chacko, S. and Eisenberg,
E. (1990). Cooperativity of gizzard acto-HMM ATPase in the presence
of gizzard tropomyosin. J. Biol.Chem.265:2105-2110.
Samuel, M., Chowrashi, PK., Pepe, FA.,
and Chacko, S. (1990) Effects of phosphorylation,
magnesium, and filament assembly on actin-activated ATPase of pig
urinary bladder myosin. Biochemistry 29:7124-3132.
Horiuchi, KY., Samuel, M., and Chacko,
S. (1991) Mechanism for inhibition of acto-HMM ATPase by
the actin/calmodulin binding domain of caldesmon. Biochemistry 30:712-717.
Horiuchi, KY., and Chacko,
S. (1991) The mechanism for the inhibition of actin-activated
ATPase of smooth heavy meromyosin by calponin. Biochem. Biophys.
Res. Commun. 176:1487-1493.
Chacko, S., Jacob,
S., Horiuchi, K. (1994) Myosin I from mammalian smooth muscle is
regulated by caldesmon-calmodulin. J. Biol. Chem. 269:1583-15807.
Wang, Z., Horiuchi, KY., Jacob, SJ.,
Gopalakurup, SK, and Chacko, S. (1994) Overexpression,
purification, and characterization of full-length and mutant caldesmon
using a baculovirus expression system. J. Musc. Res. & Cell
Motil. 15: 646-658.
Horiuchi, KY. and Chacko, S.
(1995) Effect of unphosphorylated smooth muscle on caldesmon-mediated
regulation of actin filament velocity. J. Musc. Res. & Cell
Motil. 16:11-19.
Horiuchi, KY., Wang, Z., and Chacko,
S. (1995) Inhibition of smooth muscle actomyosin ATPase
by caldesmon is associated with caldesmon-induced conformational
changes in tropomyosin bound to actin. Biochemistry.34: 16815.
Wang, Z. , Horiuchi, KY., and Chacko,
S. (1996) Characterization of the functional domains of
the C-terminal region of caldesmon using full-length and mutant
caldesmon molecules. J. Biol. Chem. 271:271:2234-2242.
Wang, Z. and Chacko, S.
(1996) Mutagenesis analysis of functionally important domains within
the C-terminal end of smooth muscle caldesmon. J. Biol. Chem. 27:25707-25714.
DiSanto, ME., Cox, RH., Wang, Z., and
Chacko, S. (1997) Increased expression of myosin
II with insertion in the NH2-terminal region of the distributing
(muscular) arteries. Amer. J.Physiol. 272(Cell) C1532-C1542.
Wang, Z., Yang, Z-Q., and Chacko,
S. (1997) Functional and structural relationship between
the calmodulin-binding, actin-binding, and actomyosin-ATPase inhibitory
domains on the C terminus of smooth muscle caldesmon .J. Biol. Chem.272:
16896-16903.
Chacko, S., DiSanto,
M., Wang, Z, Zderic, SA., and Wein, AJ. (1997).Contractile protein
changes in urinary bladder smooth muscle during obstruction-induced
hypertrophy. Scand. J.Urol. Neprol. 184: 67-76.
Wang, Z., He, J., Yang, Z-Q., and Chacko,
S. (1997) Both N-terminal myosin binding and C-terminal
actin-binding sites on smooth muscle caldesmon are required for
caldesmon-mediated inhibition of actin filament velocity. Proc.
Natl Acad. Sci. 94: 11899-11904.
Menon, C., and Chacko, S.
(1998) Expression of caldesmon during differentiation of smooth
muscle in the chicken gizzard. Tissue & Cell Res. 30:118-126
DiSanto, M., Chacko, T., Hypolite,
J., Broderick, G., Wein, AJ, and Chacko, S. (1998)
Molecular Basis of Erecetile Function: Expression of myosin isoforms
in smooth muscle cells in corpus cavernosum penis. Am. J. Physiology
(Cell Physiol 44) 275:C976-C987.
Gomes, CM., DiSanto, ME., Horan, P.,
Levine, RM., Wein, AJ., and Chacko, S. (2000) Improved
contractility obstructed bladders after tadenan treatment is associated
with reversal of altered myosin isoform expression. J. Urol. 163:
2008-2013.
Hypolite, J., DiSanto, M., Zheng, Y.,
Chang, S., Wein, A., and Chacko, S. (2001) Regional
variation on myosin isoforms and phosphorylation at the resting
tone in urinary bladder smooth muscle. Am. J. Physiol.(Cell) 280:
C254-C264
Sanchez-Ortiz, R., Wang, Z., Menon,
C., DiSanto, ME., Wein, AJ, and Chacko, S. (2001).
Estrogen modulates the expression of myosin heavy chain in detrusor
smooth muscle. Am. J. Physiol. (Cell) 280: C433-C440.
Arafat, HA., Kim, G., DiSanto, ME.,
Wein, AJ, and Chacko, S. (2001) Heterogeneity of
bladder myocytes in vitro: modulation of myosin isoform expression.
Tissue & Cell. 33 (3) 1-14.
Chang, S., Hypolite, J., Zderic, SA.,
Wein, AJ., Chacko, S., DiSanto, ME (2002) Enhanced
force generation by corpus cavernosum smooth muscle in rabbits with
partial bladder outlet obstruction. J. Urol 167:2636-2644.
Zheng, Y., Weber, W.T., Wang, S., Wein,
A.J., Chacko, S., and DiSanto, M.E. (2002) Establishment
of a phenotypically stable bladder myocyte cell line from hypertrophied
detrusor smooth muscle. Am. J. Physiol Cell Physiology 283:C373-382.
Chang, S., Hypolite, J., Changolkar,
A., Wein, AJ., Chacko, S., DiSanto, ME (2003) Increased
contractility of diabetic rabbit copora smooth muscle in response
to endothelin is mediated via Rho-kinase ß. Int J Impot Res
15: 53-62.
DiSanto ME, Stein R, Chang S, Hypolite
JA, Zheng Y, Zderic S, Wein AJ, Chacko S. (2003)
Alteration in the expression of myosin isoforms in detrusor smooth
muscle following bladder outlet obstruction. Am J Physiol Cell Physiol.,
285(6):C1397-410.
Bing W, Chang S, Hypolite JA, DiSanto
ME, Zderic SA, Rolf L, Wein AJ, Chacko S. (2003)
Obstruction-induced changes in urinary bladder smooth muscle contractility:
A role for Rho-kinase. Am J Physiol Renal Physiol. ;285(5):F990-7.
Zhang E, Stein R, Chang S, Zheng Y,
Zderic SA, Wein AJ, and Chacko S. (2004) Smooth
Muscle Hypertrophy following Partial Bladder Outlet Obstruction
is Associated with Overexpression of Non-Muscle Caldesmon. Am J.
Pathology.,164: 601-612.
Austin JC; Chacko SK;
DiSanto M; Canning DA; Zderic SA; (2004) A male murine model of
partial bladder outlet obstruction reveals changes in detrusor morphology,
contractility and Myosin isoform expression Journal of Urology 172(0):
1524-1528.
Su X; Changolkar A; Chacko
S; Moreland RS; (2004) Diabetes decreases rabbit bladder
smooth muscle contraction while increasing levels of myosin light
chain phosphorylation Am J Physiol Renal Physiol 287(4): F690-9.
Chang S; Hypolite JA; Velez M; Changolkar
A; Wein AJ; Chacko S; DiSanto ME; (2004) Downregulation
of cGMP-dependent protein kinase-1 activity in the corpus cavernosum
smooth muscle of diabetic rabbits Am J Physiol Regul Integr Comp
Physiol 287(4): R950-60.
Chacko S.; Chang S;Hypolite
J;DiSanto ME;Wein AJ; (2004) Alteration of Contractile and Regulatory
Proteins Following Partial Bladder Outlet Obstruction. Scandinavian
Journal of Urology & Nephrology, 215:26-36.
Chang S, Hypolite JA, Wein AJ, Chacko
S, Disanto ME (2005) Increased corpus cavernosum smooth
muscle tone associated with partial bladder outlet obstruction is
mediated via Rho-kinase. Am J Physiol Regul Integr Comp Physiol.;289(4):R1124-30.
Changolkar A; Hypolite J; DiSanto
ME; Oates P; Wein AJ; Chacko S; (2005) Diabetes-Induced
Decrease in Detrusor Smooth Muscle Force is Associated with Oxidative
Stress and Overactivity of Aldose Reductase. Journal of Urology
173:309-313.
Mannukarottu, AS., Hypolite, JA., Zderic.
SA., Wein, AJ., Chacko, S., DiSanto, ME. (2005)
Regional Alterations in the Expression of Smooth Muscle Myosin Isoforms
in Response to Partial Bladder Outlet Obstruction. Journal of Urology
173:302-308.
Chang S, Hypolite JA, Disanto ME, Changolkar
A, Wein AJ, Chacko S (2005) Increased basal phosphorylation
of detrusor smooth muscle myosin in Alloxan-induced diabetic rabbit
is mediated by up-regulation of Rho-kinase {beta} and CPI-17. Am
J Physiol Renal Physiol. 290(3):F650-6.
Mannukarottu, AS., DiSanto, ME., Zderic.
SA., Wein, AJ., Chacko, S (2005) Altered expression
of thin filament-associated proteins in hypertrophied urinary bladder
smooth muscle. Neurourol Urodyn. 25(1):78-88.
Mannukarottu, AS., DiSanto, ME., Zderic,
SA., Wein, AJ., Chacko, S (2006)Altered expression
of thin filament-associated proteins in hypertrophied urinary bladder
smooth muscle. Neurourol Urodyn. 25(1):78-88.
Hypolite JA, Chang S, Zheng Y, DiSanto
ME, Zderic SA, Wein AJ, Chacko S (2006) Partial
bladder outlet obstruction induces urethral smooth muscle hypertrophy
and decreased force generation. J Urol. 175(2):777-82.
Macarak, EJ., Schultz, J., Zderic,
SA., Sado, Y., Polyak, E., Chacko, S, and Howard,
PS. (2006) Smooth muscle trans-membrane sarcoglycan complex in partial
bleadder ouitlet obstruction. Histochemistry. Cell Biol. 2006 Jan
25;1-12.
LaBelle E., Zderic, SA., Delaney, D.,
Hypolyte, J., Wein, A.J. and Chacko, S. (2006)Lipid
signaling changes in smooth muscle remodeling associated with partial
urinary bladder outlet obstruction. Neurourol Urodyn.;25(2):179-84.
Basha, M., Chang, S., Somolock, E.M.,
Moreland, R.S., Wein, A.J., and Chacko S. (2006)
Regional differences in myosin heavy chain isoform expression and
maximal shortening velocity of rat vaginal wall smooth muscle. Am.
J. Physiol. Regul Integr. Comp. Physiol 291(4):R1076-84.
Stanton MC, Austin JC, Delaney DP,
Gosfield A, Marx JO, Zderic SA, Chacko S, Moreland
RS. (2006) Partial bladder outlet obstruction selectively abolishes
protein kinase C induced contraction of rabbit detrusor smooth muscle.
J Urol, 176(6 Pt 1):2716-21.
Deng M, Mohanan S, Polyak E, Chacko
S.(2007) Caldesmon is necessary for maintaining the actin
and intermediate filaments in cultured bladder smooth muscle cells.
Cell Motil Cytoskeleton, 64(12):951-65.
Hypolite JA, Chang S, LaBelle, E, Babu,
G., Periasamy M, Wein AJ, and Chacko S (2008) Deletion
of SM-B, the high ATPase isoform of myosin, upregulates the PKC-mediated
signal transduction pathway in murine urinary bladder smooth muscle.
Am J Physiol Renal Physiol. 296: F658-F665, 2009. First published
December 3, 2008; doi:10.1152/ajprenal.90221.2008.
Chang S, Hypolite JA, Mohanan S, Zderic
SA, Wein AJ, Chacko S. (2009) Alteration of the
PKC-mediated signaling pathway for smooth muscle contraction in
obstruction-induced hypertrophy of the urinary bladder. Lab Invest.
89(7):823-32.
Basha M, Labelle EF, Northington GM,
Wang T, Wein AJ, Chacko S. (2009) Functional significance
of muscarinic receptor expression within the proximal and distal
rat vagina. Am J Physiol Regul Integr Comp Physiol. 297(5):R1486-93.
Polyak E, Boopathi E, Mohanan S, Deng
M, Zderic SA, Chacko S. (2009) Alteration of the
caveolin expression and ultrastructure in bladder smooth muscle
hypertrophy. J Urol. 182(5):2497-503.
Daneshgari F, Liu G, Birder L, Hanna-Mitchell
AT, Chacko S. (2009). Diabetic bladder dysfunction:
current translational knowledge. J Urol. 182(6 Suppl):S18-26. Review.
Chang S, Gomes CM, Hypolite JA, Marx
J, Alanzi J, Zderic SA, Malkowicz B, Wein AJ, Chacko S.
(2010). Detrusor overactivity is associated with the down-regulation
of large conductance calcium- and voltage-activated potassium channel
protein. Am J Physiol Renal Physiol. 298(6):F1416-23.
Pan XQ, Gonzalez JA, Chang S, Chacko
S, Wein AJ, Malykhina AP. (2010). Experimental colitis
triggers the release of substance P and calcitonin gene-related
peptide in the urinary bladder via TRPV1 signaling pathways. Exp
Neurol. PMID: 20501335. |