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Neurourology Research Laboratory
Harrison Department of Surgical Research
Anna P. Malykhina, Ph.D.

Equipment in laboratories includes two setups for patch-clamp recordings with the Ti inverted microscope with fluorescent filters (Nikon, Japan), digital camera (Nikon, Japan), Axoclamp 200B amplifier (Axon Instrument), Digidata 1440 (Axon Instruments), microforge (Narishige, Japan) and puller (Sutter Instruments) for making electrodes. There are several full sets of surgical instruments for survival rodent surgeries. Separate room in the Glenolden animal facility is fully equipped for survival rodent surgeries including table-top anesthesia machine (LEI Medical Inc). Molecular biology lab consists of Nuaire Tissue culture hood, Jouan incubator, table top centrifuge for eppendorf tubes, water bath; Sorvall Legend Mach 1.6R refrigerated centrifuge (ThermoScientific), Robocycler gradient PCR machine (Stratagene), tissue homogenizer and sonicator, electrophoresis equipment for RNA and DNA, Biodoc system for gel analysis, 4 C refrigerators (TermoScientific), -20 C and -80 C freezers.

Basic Urological Research directed by Dr. Samuel K. Chacko provides access to the core facility within the same building which include the areas for organ bath contractile studies, cystometry measurements as well as imaging core facility. The core facility of Basic Urologic Research is also equipped with Packard liquid scintillation analyzer for beta and gamma counting, Sorvall RC-70 ultracentrifuge, Sorvall RC-5B high speed centrifuge, JEC refrigerated bench top centrifuge, Berthold luminescence spectrometer, Hitachi spectrophotometer, glass and Teflon hand homogenizers, Brandel cell harvester for binding assays, several circulating water baths with and without shakers, autoclave, chromatography columns of several types with fraction collectors, refrigerators and freezers, analytical balances, conventional drying oven, ice machine, water deionizing system. Basic Urologic Research core facility also provides equipment for physiological, biochemical, and molecular biological studies. These facilities include equipments for electrophoresis, isoelectric focusing with column and mini platform (BioRad) as well as units for Northern, Southern, and Western blotting. There is also physiological equipment available for measuring contractile force in vitro (organ bath setup) which includes a Grass model 7D polygraph and a Grass model S-88 electrical stimulator.


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