penn medicine
penn surgery
penn surgery home penn surgery clinical penn surgery education penn surgery research penn surgery administration

Alan W. Flake, M.D.
Pediatric Surgery

 
  • Profile Home
  • Appointments
  • Education
  • Societies
  • Research
  • Publications
  • Awards

Administrative Office:
Children’s Hospital of Philadelphia
Abramson Research Center, Rm. 1116B
3615 Civic Center Blvd.
Philadelphia, PA 19104
Administrative Assistant: Colleen Corcoran
Administrative Telephone: 215-590-3671
Administrative Fax: 215-590-3324
Patient Appointments: 215-590-2744

Clinical Offices:
Childrenís Hospital of Philadelphia
Abramson Research Center, Rm. 1102D
3516 Civic Center Blvd.
Philadelphia, PA 19104
Clinical Telephone: 215-590-2744
Clinical Fax: 215-590-3240
Patient Appointments: 215-590-2744

Contact Information (internal use only)

flake@email.chop.edu

Link to Children's Hospital of Philadelphia

 

Alan W. Flake, M.D. is Director of the Children’s Institute for Surgical Science at the Children’s Hospital of Philadelphia and is a Professor of Surgery and Obstetrics and Gynecology at the University of Pennsylvania School of Medicine. He maintains an active surgical practice in pediatric general, and thoracic surgery and has a focused clinical and research interest in fetal surgery. He is director of an active research laboratory located on the 11th floor of the Abramson Research Building investigating fetal stem cell and gene therapy, as well as prenatal correction of specific fetal surgical anomalies.

 
Faculty Appointments
2002 - present Professor of Surgery and Obstetrics, and Gynecology
University of Pennsylvania
 
1998 - 2012 Pediatric Surgery Fellowship Training Director
Children’s Hospital of Philadelphia
 
1997 - present Full Member, Institute of Human Gene Therapy, University of Pennsylvania
 
1996 - present Senior Member, Joseph Stokes, Jr. Research Institute
 
1996 - present Director of the Children’s Institute for Surgical Science
re-named the Center for Fetal Research (2006)
Stokes Research Institute, Abramson Pediatric Research Center
 
1996 - 2001 Associate Professor of Surgery and Obstetrics, and Gynecology
University of Pennsylvania, Ruth and Tristram C. Colket, Jr. Professor of Pediatric Surgery, Children's Hospital of Philadelphia
 
1994 - 1996 Director of Fetal Surgery, Children’s Hospital of Michigan
 
1994 - 1996 Associate Professor of Surgery and Obstetrics and Gynecology
Wayne State University, Children’s Hospital of Michigan, Detroit, Michigan  
 
1990 - 1994  Assistant Professor of Surgery and Pediatrics, Division of Pediatric Surgery
University of California, San Francisco
 
Hospital and Administrative Appointments
1997 - 2003

Consulting Staff, Department of Surgery
The Chester County Hospital
West Chester, Pennsylvania

 
1997 - present Courtesy Staff, Department of Surgery
Pennsylvania Hospital, Philadelphia, Pennsylvania
 
1997 - present Active Clinical Staff, Hospital of the University of Pennsylvania
Philadelphia, Pennsylvania
 
1997 - present Pediatric Surgery Fellowship Training Director
Children’s Hospital of Philadelphia
Philadelphia, Pennsylvania
 
1996 - present Active Clinical Staff, Children’s Hospital of  Philadelphia
Philadelphia, Pennsylvania
 
1994 - 1996 Active Clinical Staff, St. John's Hospital
Detroit, Michigan
 
1994 - 1996  Active Clinical Staff, Hutzel Hospital, Detroit Medical Center
Detroit,  Michigan
 
1994 - 1996 Active Clinical Staff, Henry Ford Hospital
Detroit, Michigan
 
1994 - 1996 Active Clinical Staff, Children's Hospital of Michigan
Director of Fetal Surgery Children's Hospital of  Michigan
Detroit, Michigan
 
1990 - 1994 Courtesy Staff, Kaiser Permanente Hospital
San Francisco, California     
 
1990 - 1994 Active Clinical Staff, California Pacific Medical Center
California and  Pacific Campuses
San Francisco, California
 
1990 - 1994  Active Clinical Staff - University California
San  Francisco Hospitals
San Francisco, California
 
Education/Training
1981 M.D., University of Arkansas for Medical Sciences
Little Rock, Arkansas
 
1977 B.S., University of Arkansas
Fayetteville, Arkansas    
 
1973 H.S., Taipei American School
Taipei, Taiwan
 
Postgraduate Training and Fellowship Appointments
1988 - 1990 Pediatric Surgery Fellowship, Children’s Hospital Medical Center
Cincinnati, Ohio
 
1986 - 1988 General Surgery PGY 4-5, University of California
San Francisco, California
 
1984 - 1986 U.C.S.F. Fetal Biology and Therapy Research Fellowship
San Francisco, California
 
1982 - 1984 General Surgery PGY 2-3, University of California
San Francisco, California
 
1981 - 1982 Intern in Surgery, University of California
San Francisco, California
 
Memberships/Societies
 
National or International Societies
2011 - present International Pediatric Endosurgery Group
 
2005 - present American Society for Blood and Marrow Transplantation
 
1999 - present International Society of Experimental Hematology
 
1998 - present American Society of Hematology
 
1997 - present American Society of Gene and Cellular Therapy
 
1996 - present International Society for Prenatal Diagnosis
 
1996 - present American Academy of Pediatrics, Surgical Section
 
1993 - present Fellow American College of Surgeons
 
1993 - present American Pediatric Surgical Association
 
1991 - present Cell Transplant Society
 
1991 - 1993 Associate Fellow American College of Surgeons
 
1992 -1994 Councilman Association for Academic Surgery
 
1991 - present Associate for Academic Surgery
 
1990 - present American Association for the Advancement of Science
 
1989 - present Extracorporeal Life Support Organization
 
1986 - 1989 Candidate Group American College of Surgeons
 
1986 - present International Fetal Medicine and Surgery Society
 
Local Societies
1998 - present American College of Surgeons - Philadelphia Chapter
 
1997 - present John Morgan Society
 
1997 - present Philadelphia Perinatal Society
 
1995 - 1998 Michigan State Medical Society
 
1995 - 1998 Wayne State Medical Society
 
1992 - 1996 San Francisco Surgical Society
 
1988 - present Howard C. Naffziger Surgical Society
 
Research
 
Grants as Principal Investigator
2004 - 2005 Development of a Large Animal Preclinical Model for In Utero Hematopoietic Cell Transplantation – Pennsylvania State Health Research Formula Award (CHOP allocation)
 
2004 - 2009 Fetal Biology and TherapyTraining Program – NIH-NICHD
 
2007 - 2010 Strategies  for Prenatal Stem Cell Transplantation  - National Institute of Health, Heart, Lung, and Blood Institute
 
2003 - 2007  Fetal Stromal Progenitor Cells - Agency - National Institute of Health, Heart, Lung, and Blood Institute
 
2003 - 2008 Sickle Cell Center Grant  (Ohene-Frempong PI); Project B3. Fetal Tolerance, Chimerism, and Sickle Cell Disease – National Institute of Health, Heart, Lung, and Blood Institute
 
2004 - 2005 Forderer Fund – Development of a Retinoic Acid induced Rat Model of Myelomeningocele
 
  Human Application of AAV-mediated muscle –directed factor IX gene transfer for hemophilia B. Project 4. National Institute of Health, Heart Lung and Blood Institute. (Alan Cohen) Co-investigator
 
2001 - 2006 Twin-Twin Transfusion Syndrome Trial – NIH-NICHD – RO1-HD41149 Co-Investigator (Crombleholme) 
 
2004 - 2006 Development  of Surrogate Human Immune System/Fetal Mouse – NIH-NIAID U19 AI057234 “Human Dendritic Cells and In Vivo Immunity to Biothreat” (PI Jacques Banchereau, Baylor Research Institute) Pilot Project
 
2001 - 2004 Strategies for Prenatal Treatmen t of Muscular Dystrophy– Muscular Dystrophy Association – Period of funding
 
1999 - 2003 Prenatal Strategies for Treatment of Hemoglobinopathies. Agency - National Institute of Health, Heart, Lung, and  Blood Institute
 
1999 - 2002 The Role of Mesenchymal Stem Cells in Fetal Tissue Engineering – Center for Innovative Minimally Invasive Therapy and the Department of Defense. Period of funding . Co-Principal Investigator with N. Scott Adzick.
 
1998 - 2000 Fetal Intramuscular Administration of Adenovirus. Pilot grant provided by the Muscular Dystrophy Association to the The Institute for Human Gene Therapy, University of Pennsylvania
 
1996 - 1998 Characterization of Mechanical and Biochemical Modulators of Fetal Lung  Growth.  National Institute of Health, Child Health and Human Development, Perinatology Branch
 
1996 - 1997 Mechanism of Lung Growth Induced by Prenatal Tracheal Occlusion, Children’s Hospital of Michigan Pilot Grant
 
1994 - 1995 In Utero Hematopoietic Stem Cell Transplantation, Children’s Hospital of Michigan Program Grant
 
1996 - 1998 In-Utero Stem Cell Transplantation in Mice. Agency - National Institute of Health, Heart, Lung and Blood Institute; Grant No 5RO1 HL53998-02; Period of funding
 
1994 - 1998 In-Utero Stem Cell Transplantation in Mice. Agency - National Institute of Health, Heart, Lung, and  Blood Institute
 
1992 - 1993 The Reduction of Congenital Diaphragmatic Hernia by Tracheal Occlusion. U.C.S.F. Research Evaluation  and Allocation Committee
 
1991 - 1992 In Vivo Effects of Growth Factor on Human Hepatocytes. U.C.S.F. Research Evaluation and  Allocation Committee
 
1991 - 1992 In-Utero Specific Tolerance Induction. Academic Senate U.C.S.F.
 
1991 - 1994 Organ Transplantation after Induction of Specific Tolerance In Utero. - American College of Surgeons
 
1991 - 1994 Transplantation of Fetal Hematopoietic Stem Cells in Utero. Agency - National Institutes of Health, Heart Lung and Blood Institute
 
Publications
 
Bibliography
  1. Adzick NS, Harrison MR, Flake AW, LaBerge JM:  Development of a fetal renal function test using endogenous creatinine clearance.  Journal of Pediatric  Surgery 20:602-607, 1985.
  2. Adzick NS, Harrison MR, Flake AW, deLorimier AA:  Urinary extravasation in the fetus with obstructive uropathy.  Journal of Pediatric Surgery 20:608-615, 1985.
  3. Adzick NS, Harrison MR, Flake AW, Glick PL, Bottles K:  Automatic uterine stapling devices in fetal surgery: Experience in a primate model.  Surgical Forum XXXVI:479-81, 1985.
  4. Adzick NS, Harrison MR, Glick PL, Anderson J, Villa RL, Flake AW, Laberge JM:  Fetal  surgery in the primate. III. maternal outcome after surgery.  Journal of Pediatric Surgery 21:477-480, 1986.
  5. Flake AW, Harrison MR, Adzick NS, LaBerge JM, Warsof SL:  Fetal sacrococcygeal teratoma. Journal of Pediatric Surgery 21:563-66, 1986.
  6. Flake AW, LaBerge JM, Adzick NS, Hill AC, Harrison MR:  Auxiliary transplantation of the fetal liver. I. Development of a sheep model.  Journal of Pediatric Surgery 21:515-520, 1986.
  7. Flake AW, Villa-Troyer RL, Adzick NS, Harrison MR:  Trans-amniotic fetal feeding. III. The effect of nutrient infusion on intrauterine growth retardation. Journal of Pediatric Surgery 21:481-484, 1986.
  8. Flake AW, Harrison MR, Adzick NS, Zanjani ED:  Transplantation of fetal hematopoietic stem cells in utero: The creation of hematopoietic chimeras.  Science 233(4765):776-8, 1986.
  9. Flake AW, Harrison MR, Adzick NS, Sauer L, deLorimier AA:  Ureteropelvic junction obstruction in the fetus. Journal of Pediatric Surgery 21:1058-63, 1986.
  10. Sauer L, Glick PL, Adzick NS, Flake AW, Harrison MR:  Compensatory renal growth following nephrectomy in fetal lambs.  Surgical Forum XXXVII:648-50, 1986.
  11. Adzick NS, Hu LM, Davies P, Flake AW, Reid L, Harrison MR:  Compensatory lung growth after pneumonectomy in fetal lambs: A morphometric study.  Surgical Forum XXXVII:309-11, 1986.
  12. Kantoff PW, Gillio A, McLachlin JR, Flake AW, Eglitis MA, Moen R, Karlsson S, Kohn DB, Karson E, Zweibel JA:  Retroviral-mediated gene transfer into hematopoietic cells.Transactions of the Association of American Physicians 99:92-102, 1986.
  13. Sauer L, Harrison MR, Flake AW:  Does an expanding fetal abdominal mass produce pulmonary hypoplasia.  Journal of Pediatric Surgery 22:508-12, 1987.
  14. Harrison MR, Golbus MS, Filly RA, Anderson RL, Flake AW, Rosen M, Huff RW:  Fetal hydronephrosis: Selection and surgical repair.  Journal of Pediatric Surgery 22:556-8, 1987.
  15. Flake AW, Harrison MR, Sauer L, Adzick NS, Krummel TM, LaBerge JM:  Auxiliary transplantation of the fetal liver. II. functional evaluation of an intraabdominal model. Journal of Pediatric Surgery 22:559-65, 1987.
  16. Sauer L, Harrison Mr, Flake AW, Heyman MB, Ring EJ;   Long-term percutaneous biliary drainage in an infant with hemangioendothelioma.  Journal of Pediatric Surgery 22:606-8, 1987.
  17. Flake AW, Villa RL, Adzick NS, Harrison MR:  Trans-amniotic fetal feeding. II. A model of intrauterine growth retardation.  Journal of Pediatric Surgery 22:816-19, 1987.
  18. Flake AW, Harrison MR, Adzick NS, Zanjani ED:  Erythropoietin production by the fetal liver in the adult environment.  Blood 70:542-5, 1987.
  19. Kuhlmann RS, Warsof SL, Levy DL, Flake AW, Harrison MR.  Fetal sacrococcygeal teratoma. Fetal Therapy 2:95-100, 1987.
  20. Kantoff PW, Flake AW, Eglitis MA, Scharf S, Bond S, Gilboa E, Erlich H, Harrison MR, Zanjani ED, Anderson WF:  In utero gene transfer and expression: A sheep transplantation model. Blood 73:1066-1073, 1988.
  21. Verrier ED, Crombleholme TM, Sauer L,  Longaker M, Langer JC, Flake AW,  Dae M, Brem H, Harrison MR:  Neonatal model of heterotopic heart transplantation in pigs.  Journal of Thoracic and Cardiovascular Surgery 98:127-35, 1989.
  22. Bond SJ, Harrison MR, Slotnick RN, Anderson J, Flake AW, Adzick NS:  Cesarean delivery and hysterotomy using an absorbable stapling device.  Obstetrics and Gynecology 74:25-8, 1989.
  23. Bond SJ, Harrison MR, Schmidt KG, Silverman NH, Flake AW, Slotnick NR, Anderson RL, Warsof SL, Dyson DC.  Death due to high output cardiac failure in fetal sacrococcygeal teratoma. Journal of Pediatric Surgery 25:1287-1291, 1990.
  24. Nelson JM, Krummel TM, Haynes JH, Flood LC, Sauer L, Flake AW, Harrison MR: Operative techniques in the fetal rabbit.  Journal of Investigative Surgery 3:393-398, 1991.
  25. Ryckman FC, Flake AW, Fisher RA, Tchervenkov JI, Pedersen SH, Balistreri WF:  Segmental orthotopic hepatic transplantation as a means to improve patient survival and diminish waiting list  mortality.  Journal of Pediatric Surgery 26:422-428, 1991.
  26. Plowden JS, Kimball TR, Bensky A, Savani R, Flake AW, Warner BW, von Allmen D, Ryckman FC: The use of extracorporeal membrane oxygenation in critically ill neonates with Ebstein's anomaly.  American Heart Journal 121: 619-622, 1991.
  27. Flake AW, Shopene C, Zeigler MM:  Anti-reflux gastrointestinal surgery in the neurologically handicapped child.  Pediatric Surgery International 6:92-97, 1991.
  28. Duncan BW, Harrison MR, Flake AW, Bethel CI, Bigler ME, Roncorolo MG:  Immune response in hematopoietic chimeric rhesus monkeys. Surgical Forum XLII:373-375, 1991.
  29. Pallavicini  MG, Flake AW, Bethel C, Langlois R, Madden D, Duncan BW, Haendel S, Montoya T: Creation of human-mouse xenogeneic chimeras by the in-utero transplantation of hemopoietic cells. Transplantation Proceedings 24:542-543, 1991.
  30. Zanjani ED, Pallavicini MG, Ascencao JL, Flake AW, Resima M, MacKintosh FR, Langlois R, Stutes D, Harrison MR, and Tavassoli M.   Engraftment and long term expression of human fetal hemopoietic stem cells in sheep following transplantation in-utero.  Journal of Clinical Investigation 89:1178-1188, 1992.
  31. Jennings RW, Flake AW, Mussan G, Harrison MR, Adzick NS, Pellegrini CA:  A novel endoscopic transgastric fundoplication procedure for gastroesophageal reflux. Journal of Laparoendoscopic  Surgery 2:207-213, 1992.
  32. von Allmen D, Babcock D, Matsumoto J, Flake AW, Warner BW, Stevenson RJ, Ryckman FC.  The predictive value of head ultrasound in the ECMO candidate.  Journal of Pediatric Surgery 27:36-39, 1992.
  33. Hedrick MH, Jennings RW, MacGillivray TE, Flake AW, Lorenz HP, Estes JM, Roberts L, Adzick NS, Harrison MR:  Endoscopic catheterization of placental vessels for chronic fetal vascular access.  Surgical Forum XLIII:504-5, 1992.
  34. Zanjani ED, Pallavacini MG, Ascensao JL, Flake AW, Harrison MR, Tavassoli M.:  Human-ovine xenogeneic transplantation of stem cells in-utero. Bone Marrow Transplantation 9 Suppl 1:86-9, 1992.
  35. Zanjani ED, Ascensao JL, Flake AW, Harrison MR, Tavassoli M: The fetus as an optimal donor and  recipient of hemopoietic stem cells.  Bone Marrow Transplantation 10 Suppl 1:107-14, 1992.
  36. Rice HE, Hedrick MH, Flake AW, Donegan E, Harrison MR:  Bacterial and fungal contamination of  human fetal liver collected transvaginally for hematopoietic stem cell transplantation.  Fetal Diagnosis and Therapy 8:74-78, 1993.
  37. Hedrick MH, Rice HE, Zanjani ED, Sachs DH, Flake AW.  Creation of pig-sheep xenogeneic chimerism by the in-utero transplantation of hematopoietic stem cells.  Transplantation Science 3:23-26, 1993.
  38. Adzick NS, Harrison MR, Flake AW, Howell LJ, Golbus MS, Filly RA, and the UCSF Fetal Treatment  Program:  Fetal surgery for cystic adenomatoid malformation of the lung. Journal of Pediatric Surgery 28:806-812, 1993.
  39. Harrison MR, Adzick NS, Flake AW, Jennings RW:  The CDH two-step: a dance of necessity.  Journal of Pediatric Surgery 28:813-816, 1993.
  40. Rice HE, Flake AW, Hedrick MH, Zanjani ED, Harrison MR:  Effect of xenogeneic chimerism in a human/sheep model on natural antibody.  Journal of Surgical Research 54:355-359, 1993.
  41. Rice HE, Hendrick MH, Bealer JF, Zanjani ED, Harrison MR, Flake AW:  Placental vessel catheterization: A new technique of chronic intravenous access in fetal sheep.  Surgical Forum XLIV:644-646, 1993.
  42. Hedrick MH, Estes JM, Sullivan KM, Bealer JF, Flake AW, Adzick NS, Harrison MR: Plug the lung until it grows (PLUG):  A new method to treat congenital diaphragmatic hernia in-utero. Surgical Forum XLIV:644-646, 1993.
  43. Harrison MR, Adzick NS, Flake AW and members of the Fetal Treatment Program: Correction of             congenital diaphragmatic hernia in-utero: VI hard earned lessons.  Journal of Pediatric Surgery 28:1411-1418 1993.
  44. Hedrick MH, Jennings RW, MacGillivray TE, Rice HE, Flake AW, Adzick NS, Harrison MR.  Chronic fetal vascular access.  Lancet 342:1086-1087, 1993.
  45. Srour, EF, Zanjani ED, Cornetta K, Traycoff CM, Flake AW, Hedrick M, Brandt JE Leemhuis T, Hoffman R:  Persistence of human multilineage self-renewing lymphohematopoietic stem cells in chimeric sheep.  Blood 82:3333-3342, 1993.
  46. Bealer JF, Natuzzi ES, Buscher C, Flake AW, adzick NS, Harrison MR:  Nitric oxide synthase is deficient in the aganglionic colon from patients with hirschsprung's disease.  Pediatrics  93:647-651, 1994.
  47. Hedrick MH, Martinez M, Filly RA, Flake AW, Adzick NS, Harrison MR:  Congenital high airway obstruction syndrome (CHAOS): A potential for perinatal intervention.  Journal of Pediatric Surgery 29:271-274, 1994.
  48. Hedrick MH, Rice HE, MacGillvray TE, Bealer JF, Zanjani ED, Flake AW: Hematopoietic chimerism achieved by in-utero hematopoietic stem cell injection does not induce donor specific tolerance or renal allografts in sheep.  Transplantation 58:110-111, 1994.
  49. Zanjani ED, Flake AW, Rice HE, Hedrick MH, Tavassoli M: Long-term hematopoietic repopulating ability of xenogenic transplanted human fetal liver stem cell (HSC) in sheep.  Journal of Clinical Investigation 93:1051-1055, 1994.
  50. Hedrick MH, Estes JM, Sullivan KM, Bealer JF, Kitterman JA, Flake AW, Adzick NS, Harrison MR:  Plug the lung unit it grows (PLUG): A new method to treat congenital diaphragmatic hernia in utero. Journal of Pediatric Surgery 29:612-617, 1994.
  51. Rice HE, Flake AW, Hori T, Galy A, Verhoogen RH:  Massive thymic hyperplasia: characterization of a rare mediastinal mass.  Journal of Pediatric Surgery  29:1561-1564, 1994.
  52. Bealer JF, Natuzzi ES, Flake AW, Adzick NS, Harrison MR:  The effect of nitric  oxide on the colonic smooth muscle of patients with hirschsprung's disease.  Journal of Pediatric Surgery 29:1025-1029, 1994.
  53. Sullivan KM, Hawgood S, Flake AW, Harrison MR, Adzick NS: Amniotic fluid phospholipid analysis in the congenital diaphragmatic hernia fetus.  Journal of Pediatric Surgery 29:1020-1024, 1994.
  54. Zanjani ED, Silva MRG, Flake AW: Retention and multilineage expression of human hematopoietic stem cells in human-sheep chimeras.  Blood Cells 20:331-340, 1994.
  55. Rice HE, Emani VR, Skarsgard ER, Knazek RA, Zanjani ED, Flake AW, Harrison MR: Human fetal liver hematopoietic cell expansion with a novel biorector system. Transplantation Proceedings 26:3338-3339, 1994.
  56. Rice HE, Hendrick MH, Flake AW: In-utero transplantation of rat hematopoietic stem cells induces xenogeneic chimerism in mice.  Transplantation Proceedings 26:126-128, 1995.
  57. Bealer JF, Skarsgard ED, Hedrick MH, Meuli M, VanderWall KJ, Flake AW, Adzick NS, Harrison MR:   The PLUG (plug the lung until it grows) odyssey: adventures in experimental fetal tracheal occlusion.  Journal of Pediatric Surgery 30:361-365,  1995.
  58. Rice HE, Skarsgard ED, Emani VR, Harrison MR, Zanjani ED, Flake AW: An effective strategy for decontamination, ex vivo expansion, and storage of human fetal liver hematopoietic stem cells.  Transplantation Proceedings 26:3352-3, 1995.
  59. Bethel CAI, Steinkirchner T, Zanjani ED, Flake AW:  The stromal microenvironment of human fetal hematopoiesis; in vitro morphologic studies.  Pathobiology 62:99-103, 1995.
  60. Flake AW, Hendrick MH, Rice HE, Tavassoli M, Zanjani ED:  Enhancement of human hematopoieses by mast cell growth factor in human-sheep chimeras created by the in-utero transplantation of human fetal hematopoietic cells.  Experimental Hematology 23:252-257, 1995.
  61. Johnson MP, Corsi P, Bradford W, Hume RF, Smith C, Flake AW, Quereshi F, Evans MI.: Sequential fetal urine analysis provides greater precision in the evaluation of fetal obstructive uropathy.  American Journal of Obstetrics and Gynecology 173:59-65, 1995.
  62. Kawashima I, Zanjani ED, Almaida-Porada G, Flake AW, Zeng H, Ogawa M. CD34+ human marrow cells that express low levels of Kit protein are enriched for  long-term marrow-engrafting cells. Blood  87:4136-42, 1996
  63. Harrison MR, Adzick NS, Flake AW, VanderWall KJ, Bealer JF, Howell LJ, Farrell JA, Filly RA, Rosen MA, Sola A, Goldberg JD. Response of the hypoplastic lung to tracheal occlusion in fetuses with diaphragmatic hernia. Journal of Pediatric Surgery 31:339-348,1996
  64. Flake AW, Roncarolo M-G, Puck JM, Ameida-Porada G, Evans MI, Johnson MP, Abella EM, Harrison DD, Zanjani ED.  Treatment of X-linked Severe Combined Immunodeficiency(X-SCID) by the in utero transplantation of paternal bone marrow. New England Journal of Medicine 335:1806-1810, 1996
  65. Flake AW, Zanjani ED. In Utero Hematopoietic Stem Cell Transplantation: A Status Report. Journal of the American Medical Association 278:932-937, 1997
  66. Mason RJ, Filipi CJ, DeMeester TR, Peters JH, Lund RJ, Flake AW, Hinder RA, Smyrk TC, Bremner CG, Thompson S. A new intraluminal antigastroesophageal reflux procedure in baboons. Gastrointestinal Endoscopy. 45:283-90, 1997.
  67. Yang EY, Cass DL, Sylvester KG, Flake AW, Wilson JM, Adzick NS. Liver-dIrected fetal gene therapy using recombinant adenoviral vectors: Successful readministration in mid-gestation sheep fetuses. Surgical Forum, XLVIII:464-467, 1997
  68. Cass DL, Yang EY, Liechty KW, Quinn TM, Crombleholme TM, Flake AW, Adzick NS. Increased cell proliferation and decreased apoptosis in congenital cystic adenomatoid malformation: Insights into pathogenesis. Surgical Forum XLVIII:659-661,1997
  69. Quinn TM, Kirsch AJ, Zderic SA, Duckett JW, Howard P, Flake AW, Adzick NS, Canning DA, Hubbard A, Snyder HM, Macarak EJ. Renal effects of partial bladder outlet obstruction in the fetal lamb. Surgical Forum XLVIII:764-766, 1997
  70. Sylvester KG, Rasanen J, Kitano Y, Flake AW, Crombleholme TC, Adzick NS. Fetal tracheal occlusion reverses high vascular impedance in diaphragmatic hernia. Surgical Forum XLVIII:649-651, 1997
  71. Liechty KW, Crombleholme TM. Flake AW, Morgan MA, Kurth CD, Hubbard AM, Adzick NS: Intrapartum airway management for giant fetal neck masses: The EXIT procedure (Ex Utero IntrapartumTreatment).  American  Journal of Obstetrics and Gynecology 177:870-874, 1997.
  72. Zanjani ED, Almeida-Porada G, , Livingston AG, Flake AW, Ogawa M. Human bone marrow CD34- cells engraft in vivo and undergo multilineage expression including giving rise to CD34+ cells. Experimental Hematology, 26:353-360, 1998
  73. Flake AW, Zanjani ED. In Utero Transplantation for Thalassemia. Annals of the New York Academy of Sciences 850:300-311, 1998
  74. Sylvester KG, Rasanen J, Kitano Y, Flake AW, Crombleholme TC, Adzick NS. Tracheal occlusion reverses the high vascular impedance to flow in the fetal pulmonary circulation and normalizes it’s physiologic response to oxygen at term. Journal of Pediatric Surgery, 33:1071-1076,1998
  75. Cass DL, Yang EY, Liechty KW, Quinn TM, Crombleholme TM, Flake AW, Adzick NS. Increased cell proliferation and decreased apoptosis in congenital cystic adenomatoid malformation: Insights into pathogenesis. Journal of Pediatric Surgery, 33:1043-1047, 1998
  76. Leahey AM, Shears GJ, Smith CA, Flake AW, Sullivan KE. Successful use of extracorporeal membrane oxygenation (ECMO) as a bridge to reconstitution following BMT for SCID. Bone Marrow Transplantation 21:839-840, 1998
  77. Kim HB, Shaaban AF, Yang EY, Liechty KW, Flake AW. Microchimerism and tolerance after in utero bone marrow transplantation in mice. Journal of Surgical Research 77:1-5, 1998.
  78. Porada CD, Tran N, Eglitis M, Moen RC, Troutman L, Flake AW, Zhao Y, Anderson WF, Zanjani ED. In utero gene therapy: Transfer and long term expression of the bacterial neoR gene in sheep following direct injection of retroviral vectors into pre-immune fetuses. Human Gene Therapy 9:1571, 1998
  79. Shaaban AF, Kim HB, Milner R, Fichter C. Flake AW. Pretreatment of prenatally transplanted allogeneic marrow with VEGF and SCF delays allograft rejection in chimeric mice. Surgical Forum XLIX:380-81, 1998.
  80. Milner R, Kim HB, Shaaban AF, Fichter C, Scully M, Asakura T, Flake AW. In utero HSC transplantation in mice transgenic for human hemoglobinopathy - Selective advantage for donor red cells. Surgical Forum XLIX:471-2, 1998
  81. Kim HB, Shaaban AF, Milner R, Fichter C, Flake AW. In utero bone marrow transplantation induces tolerance by a combination of clonal deletion and anergy. Surgical Forum XLIX:353-4, 1998
  82. Yang EY, Kim HB, Shaaban AF, Milner R, Adzick NS, Flake AW. Fetal intramuscular delivery of recombinant adenovirus results in persistent postnatal transgene expression in both muscle and liver. Surgical Forum XLIX:368-9, 1998
  83. Lovvorn HN, Crombleholme TM, Drott H, Flake AW, Adzick NS. Maternal colloid osmotic pressure after fetal surgery. Surgical Forum XLIX:481-2, 1998
  84. Kitano Y, von Allmen D, Yang EY, Quinn TM, Kanai M, Adzick NS, Flake AW. Prenatal tracheal occlusion enhances lung growth in the nitrofen induced CDH model. Surgical Forum XLIX:587-9,1998
  85. Noorily MJ, Farmer DL, Flake AW. The association of complete laryngotracheoesophageal cleft with left lung agenesis: Pathophysiologic clues provided by an experiment of nature. Journal of Pediatric Surgery 33:1546-1549, 1998
  86. Adzick NS, Harrison MR, Crombleholme TM, Flake AW, Howell LJ. Fetal lung lesions: Management and outcome. American Journal of Obstetrics and Gynecology, 179:884-889,  1998
  87. Kitano Y, Yang EY, von Allmen D, Quinn TM, Adzick NS, Flake AW. Tracheal occlusion in the fetal rat: A new experimental model for the study of accelerated lung growth. Journal of Pediatric Surgery 33:1741-1745,1998
  88. Adzick NS, Sutton LN, Crombleholme TM, Flake AW. Successful fetal surgery for spina bifida.  Lancet  352(No. 9141):1675-1676, 1998
  89. Milner R, Shaaban AF, Kim HB, Fichter C, Flake AW. Postnatal booster injections increase engraftment after in utero stem cell transplantation. Journal of Surgical Research. 83, 44-47, 1999.
  90. Liechty KW, Crombleholme TM, Weiner S, Bernick B, Flake AW, Adzick NS. Use of the ex utero intrapartum treatment procedure for a large fetal neck mass in a twin gestation. Obstetrics and Gynecology 93:824-825, 1999
  91. Yang EY, Kim HB, Shaaban AF, Milner R, Adzick NS, Flake AW. Persistent postnatal transgene expression in both muscle and liver after fetal injection of recombinant adenovirus. Journal of Pediatric Surgery 34:766-773, 1999
  92. Kim HB, Shaaban AF, Milner R, Fichter C, Flake AW. In utero bone marrow transplantation induces tolerance by a combination of clonal deletion and anergy. Journal of Pediatric Surgery 34:726-730, 1999
  93. Quinn TM, Sylvester KG, Kitano Y, Kitano Y, Jarrett BP, Sablich TJ, Adzick NS, Flake AW. TGF-b2 is increased after fetal tracheal occlusion. Journal of Pediatric Surgery 34:701-705, 1999
  94. Liechty KW, Quinn TM, Cass DL, Crombleholme TM, Flake AW, Adzick NS. Elevated Platelet-Derived Growth Factor-B in congenital cystic adenomatoid malformations (CCAMS) requiring fetal resection. Journal of Pediatric Surgery 34:805-810, 1999
  95. Kitano Y, Davies P, von Allmen D, Adzick NS, Flake AW. Fetal tracheal occlusion in the rat model of nitrofen induced congenital  diaphragmatic hernia. Journal of Applied Physiology 87:769-775, 1999
  96. Flake AW, Zanjani ED. In utero hematopoietic stem cell transplantation. Ontologic opportunities and biologic barriers. Blood 94:2179-2191, 1999
  97. Zanjani ED, Flake AW, Almeida-Porada G, Tran N, Papayannopoulou T. Homing of human cells in the fetal sheep model: Modulation of antibodies activating or inhibiting Very Late Activation Antigen-4 dependent function. Blood 94:2515-2522, 1999
  98. Milner R, Shaaban AF, Kim HB, Flake AW. Increased donor cell engraftment after in utero stem cell transplantation with postnatal booster injections in an allogeneic strain combination. Surgical Forum L:406-407, 1999
  99. Shaaban AF, Kim HB, Milner R, Flake AW. Fetal liver is a superior donor source for the engraftment of prenatally transplanted allogeneic hematopoietic stem cells. Surgical Forum L:408-411, 1999
  100. Walsh DS, Long T, Olutoye O, Crombleholme TM, Flake AW, Adzick NS.  A new minimally invasive technique for fetal tracheal occlusion in congenital diaphragmatic hernia. Surgical Forum L:504-506, 1999.
  101. Kitano Y, Radu A, Shaaban AF, Flake AW. Selection and expansion of early-cycling mesenchymal progenitors from murine bone marrrow by retroviral transduction. Surgical Forum L:550-552. 1999
  102. Kanai M, Kitano Y, Radu A, Adzick NS, Flake AW, von Allmen D. Effect of prenatal tracheal occlusion on pulmonary arterial structural changes in rats with nitrofen induced congenital diaphragmatic hernia. Surgical Forum L:567-568, 1999
  103. Madihally SV, Flake AW, Matthew HWT. Maintenance of CD34 expression during proliferation of CD34(+) cord blood cells on glycosaminoglycan surfaces. Stem Cells 17:295-305, 1999
  104. Shaaban AF, Kim HB, Milner R, Flake AW.  A kinetic model for homing and migration of prenatally transplanted marrow. Blood 94, 3251-3257, 1999
  105. Sutton LN, Adzick NS, Bilanuk LT, Johnson MP, Crombleholme TM, Flake AW. Improvement in hindbrain herniation demonstrated by serial fetal magnetic resonance imaging following fetal surgery for myelomeningocele. Journal of the American Medical Association 282:1826-1831, 1999
  106. Almeida-Porada G, Flake AW, Glimp HA, Zanjani ED. Co-transplantation of stroma results in enhancement of engraftment and early expression of donor hematopoietic stem cells in utero. Experimental Hematology 27:1569-1575, 1999
  107. Hubbard AM, Crombleholme TM, Adzick NS, Coleman BG, Howell LJ, Meyer JS, Flake AW. Perinatal MRI evaluation of congenital diaphragmatic hernia. American Journal of Perinatology 16:407-413, 1999
  108. Kitano Y, Flake AW, Quinn TM, Kanai M, Davies P, Sablich TJ, Schneider C, Adzick NS, von Allmen D. Lung growth induced by tracheal occlusion in the sheep is augmented by airway pressurization. Journal of Pediatric Surgery 35:216-222, 2000
  109. Milner R, Kitano Y, Oluyinka O, Flake AW, Adzick NS. Radiofrequency thermal ablation (RTA): A potential treatment for hydropic fetuses with a large chest mass. Journal of Pediatric Surgery 35:386-389, 2000
  110. Kay MA, Manno CS, Ragni MV, Larson PJ, Couto LB, McClelland A, Glader B, Chew AJ, Tai SJ, Herzog RW, Arruda V, Johnson F, Scallan C, Skarsgard E, Flake AW, High KA. Evidence for gene transfer and expression of blood coagulation factor IX in patients with severe hemophilia B treated with an AAV vector. Nature Genetics 24:257-261, 2000
  111. Pameijer CR, Hubbard AM, Coleman B, Flake AW. Combined pure esophageal atresia, duodenal atresia, biliary atresia, and pancreatic ductal atresia: Prenatal diagnostic features and review of the literature. Journal of Pediatric Surgery 35:745-747, 2000
  112. Crombleholme TM, Sylvester KG, Flake AW, Adzick NS: Salvage of a fetus with congenital high airway obstruction syndrome (CHAOS). Fetal Diagnosis and Therapy 15:280-282, 2000.
  113. Liechty KW, MacKenzie TC, Shaaban AF, Radu A, Moseley AB, Deans R, Marshak DR, Flake AW. Human mesenchymal stem cells engraft and demonstrate site-specific differentiation after in utero transplantation in sheep. Nature Medicine 6:1282-1286, 2000.
  114. Flake AW, Crombleholme TM, Johnson MP, Howell LJ, Adzick NS. Treatment of severe congenital diaphragmatic hernia by fetal tracheal occlusion: Clinical experience with fifteen cases. American Journal of Obstetrics and Gynecology 183:1059-1066, 2000
  115. Walsh DS, Hubbard AM, Olutoye OO, Howell TM, Flake AW, Johnson MP, Adzick NS. Assessment of fetal lung volumes and liver herniation with magnetic resonance imaging in congenital diaphragmatic hernia. American Journal of Obstetrics and Gynecology 183:1067-1069, 2000
  116. Mahle WT, Rychik J, Tian ZY, Cohen MS, Howell LJ, Crombleholme TM, Flake AW, Adzick NS. Echocardiographic evaluation of the fetus with a congenital cystic adenomatoid malformation. Ultrasound in Obstetrics and Gynecology 16:620-624, 2000
  117. Kitano Y, Radu A, Shaaban AF, Flake AW. Selection, enrichment and culture expansion of murine mesenchymal progenitor cells by retroviral transduction of cycling adherent bone marrow cells. Experimental Hematology, 28:1460-1469, 2000
  118. Correia-Pinto J, Tavares ML, Baptista MJ, Estevão-Costa J, Flake AW, Leite-Moreira AF. A new fetal rat model of gastroschisis: Development and early characterization. Journal of Pediatric Surgery 36:213-216, 2000
  119. Hoydu AK, Kitano Y, Kriss A, Hensley H, Bergey B, Flake A, Hubbard A, Leigh JS. In vivo, in utero, microscopic magnetic resonance imaging of rat fetuses with nitrofen induced congenital diaphragmatic hernia. Magnetic Resonance in Medicine 44:331-335, 2000
  120. Kitano Y, Kanai M, Davies P, von Allmen D, Yang EY, Radu A, Kitano Y, Adzick NS, Flake AW. BAPS Prize 1999: Lung growth induced by prenatal tracheal occlusion and its modifying factors: A study in the rat model of congenital diaphragmatic hernia. Journal of Pediatric Surgery 36:251-259, 2001
  121. Kitano Y, von Allmen D, Kanai M, Quinn TM, Davies P, Kitano Y, Flake AW. Fetal lung growth after short term tracheal occlusion is linearly related to intratracheal pressure. Journal of Applied Physiology 90:493-500, 2001
  122. Kanai M, Kitano Y, von Allmen D, Davies P, Adzick NS, Flake AW. Fetal tracheal occlusion in the rat model of nitrofen-induced congenital diaphragmatic hernia: Tracheal occlusion reverses the arterial structural abnormality. Journal of Pediatric Surgery 36:839-845, 2001
  123. MacKenzie TC, Howell LJ, Flake AW, Adzick NS. The management of prenatally diagnosed choledochal cysts. Journal of Pediatric Surgery  36:1241-1243, 2001
  124. MacKenzie TC, Shaaban AF, Radu A, Flake AW. Treatment of muscular dystrophy by in utero stem cell transplantation. Surgical Forum LII:328-29,  2001
  125. Flake AW. Prenatal Intervention: Ethical Considerations for Life-Threatening and Non-Life-Threatening Anomalies. Seminars in Pediatric Surgery 10:212-221, 2001
  126. Austin JC, Canning DA, Johnson MP, Flake AW, Carr MC. Vesicoamniotic shunt in a female fetus with prune belly syndrome. Journal of Urology 166:2382-2383, 2001
  127. MacKenzie TC, Flake AW. Multilineage Differentiation of Human MSC after In Utero Transplantation.  Cytotherapy 3:403-405, 2001
  128. Arruda VR, Fields PA, Milner R, Wainwright L, De Miguel MP, Donovan PJ, Herzog RW, Nichols TC, Biegel JA, Razavi M, Dake M, Huff D, Flake AW, Couto L, Kay MA, High KA.Lack of Germline Transmission of Vector Sequences Following Systemic Administration of Recombinant AAV-2 Vector in Males. Molecular Therapy 6:586-592, 2001.
  129. Correia-Pinto J, Tavares ML, Baptista MJ, Henriques-Coelho T, Estevão-Costa J, Flake AW, Leite-Moreira AF. Meconium dependence of bowel damage in gastroschisis. Journal of Pediatric Surgery 37:31-36, 2002.
  130. Krupnick AS, Kreisel D, Engels FH, Szeto WY, Plappert T, Popma SH, Flake AW, Rosengard BR. A novel small animal model of left ventricular tissue engineering. Journal of Heart and Lung Transplantation  21:233-43, 2002.
  131. Krupnick AS, Shaaban AF, Flake AW. Bone marrow tissue engineering. Tissue Engineering  8:145-155, 2002
  132. MacKenzie TC, Crombleholme TM, Johnson MP, Schnaufer L, Flake AW, Hedrick HL, Howell LJ, Adzick NS. The natural history of prenatally diagnosed conjoined twins.  Journal of Pediatric Surgery 37:303-309, 2002.
  133. Crombleholme TM, Coleman B, Hedrick H, Liechty K, Howell L, Flake AW, Johnson M, Adzick NS. Cystic adenomatoid malformation volume ratio predicts outcome in prenatally diagnosed cystic adenomatoid malformation of the lung. Journal of Pediatric Surgery 37:331-338, 2002.
  134. Correia-Pinto J, Reis  JL, Hutchins GM, Baptista MJ, Flake AW,  Leite-Moreira AF. In utero meconium exposure increases spinal cord necrosis in a rat model of myelomeningocele. Journal of Pediatric Surgery 37:488-492, 2002
  135. Bouchard S, Johnson MP, Flake AW, Adzick NS, Crombleholme TM: The EXIT procedure: experience and outcomes in 31 cases. Journal of Pediatric Surgery 37:418-426, 2002.
  136. MacKenzie TC,  Shaaban AF, Radu A, Flake AW. Engraftment of bone marrow and fetal liver cells after in utero transplantion in mdx mice. Journal of Pediatric Surgery 37:1058-1064, 2002
  137. Cohen MS, Rychik J, Bush DA, Tian ZY, Howell LJ, Long T, Adzick NS, Flake AW,  Johnson MP, Spray TL, Crombleholme TC. Influence of congenital heart disease on survival in children with congenital diaphragmatic hernia Journal of Pediatrics 141:25-30, 2002
  138. Hayashi S, Peranteau W, Shaaban AF, Flake AW. Complete allogeneic hematopoietic chimerism achieved by a combined strategy of in utero hematopoietic stem cell transplantation and postnatal donor lymphocyte infusion. Blood 100:804-812, 2002
  139. MacKenzie TC, Kobinger GP, Kootstra NA, Radu A, Sena-Esteves M, Bouchard S,Wilson JM, Verma IM, Flake AW. Efficient transduction of liver and muscle after in utero injection of lentiviral vectors with different pseudotypes. Molecular Therapy  6:349-358, 2002 
  140. Davey MG, Hedrick HL, Kanai M, Mendoza JM, Adzick NS, Flake AW. Pulmonary epithelial liquid absorption, Expressed in relation to alveolar surface area, is reduced in fetal lambs following in utero tracheal occlusion. Pediatric Pulmonology 34:278-286, 2002 
  141. Peranteau WH, Hayashi S, Hsieh M, Shaaban AF, Flake AW. High level allogeneic chimerism achieved by a strategy of prenatal tolerance induction and postnatal non-myeloablative bone marrow transplantation. Blood 100:2225-2234, 2002
  142. Hedrick HL, Flake AW, Crombleholme TM, Howell LJ, Johnson MP, Wilson RD, Adzick NS. The History of Fetal Diagnosis and Therapy: The Children’s Hospital of Philadelphia Experience. Fetal Diagnosis and Therapy 18:65-82, 2003
  143. Davey MG, Hedrick HL, Bouchard S, Adzick NS, Flake AW. Temporary tracheal occlusion in fetal sheep with severe lung hypoplasia does not improve postnatal respiratory function. Journal of Applied Physiology 94:1054-1062, 2003
  144. Hayashi S, Abdulmalik O, Peranteau WH, Ashizuka S, Campagnoli C, Chen Q, Horiuchi K, Asakura T, Flake AW. Mixed chimerism following in utero hematopoietic stem cell transplantation in murine models of hemoglobinopathy. Experimental Hematology 31:176-184, 2003
  145. Vannucchi MG, Midrio P, Flake AW, Faussone-Pellegrini MS. Neuronal differentiation and myenteric plexus organization are delayed in gastroschisis. An immunohistochemical study in a rat model. Neuroscience Letters  339:77-81, 2003
  146. Davey MG, Hedrick HL, Bouchard S. Adzick NS, Flake AW, Doolin EJ. Computer-assisted sterology: point fraction of lung parenchyma and alveolar surface density in fetal and newborn sheep. Scanning 25:37-44,  2003
  147. Manno CS, Chew AJ, Hutchison S, Larson PJ, Herzog RW, Arruda VR, Tai SJ, Ragni MV, Thompson A, Ozelo M, Couto LB, Leonard DG, Johnson FA, McClelland A, Scallan C, Skarsgard E, Flake AW, Kay MA, High KA, Glader B. AAV-mediated factor IX gene transfer to skeletal muscle in patients with severe hemophilia B. Blood. 101:2963-2972, 2003
  148. Keswani SG, Johnson MP, Adzick NS, Hori S, Howell LJ, Wilson RD, Hedrick H, Flake AW, Crombleholme TM. In utero limb salvage: Fetoscopic release of amniotic bands for threatened limb ischemia. Journal of Pediatric Surgery 38:848-851, 2003
  149. Lim F-Y, Crombleholme TM, Hedrick HL, Flake AW, Johnson MP, Howell LJ, Adzick NS. Congenital high airway obstruction syndrome: Natural history and management. Journal of Pediatric Surgery  38:940-945, 2003
  150. Kitano Y, von Allmen D, Kanai M, Adzick NS, Flake AW. Epinephrine Inhibits Tracheal Occlusion Induced Lung Growth in Fetal Sheep. Fetal Diagnosis & Therapy 18:333-337, 2003.
  151. Wilson RD, Johnson MP, Crombleholme TM, Flake AW, Hedrick HL, King M, Howell LJ, Adzick NS. Chorionic membrane separation following open fetal surgery: Pregnancy outcome. Fetal Diagnosis & Therapy 18:314-320, 2003
  152. Johnson MP, Sutton LN, Rintoul N, Crombleholme TM, Flake AW, Howell LJ, Hedrick HL, Wilson RD, Adzick NS. Fetal myelomeningocele repair: Short term clinical outcomes. American Journal of Obstetrics and Gynecology 189:482-487, 2003
  153. Correia‑Pinto J, Baptista MJ, Pedrosa C, Estevão-Costa  J, Flake AW, Leite-Moreira AF. Fetal Heart Development in the Nitrofen-Induced CDH Rat Model. The role of mechanical and non-mechanical factors. Journal of Pediatric Surgery 38:1444-1451,  2003
  154. Olutoye OO, Johnson MP, Coleman BG, Crombleholme TM,  Adzick NS, Flake AW. Abnormal umbilical cord dopplers may predict impending demise in fetuses with sacrococcygeal teratoma. Fetal Diagnosis & Therapy 18:428-431, 2003
  155. Bouchard S, MacKenzie TC, Radu A, Hayashi S, Peranteau WH, Chirmule N, Flake AW. Long-term Transgene Expression in Cardiac and Skeletal Muscle Following Fetal Administration of Adenoviral or Adeno-Associated Viral Vectors in Mice. The Journal of Gene Medicine 5:941-950, 2003
  156. Hedrick HL, Crombleholme TM, Flake AW, Nance ML, Von Allmen D, Howell LJ, Johnson MP, Wilson RD, Adzick NS. Right congenital diaphragmatic hernia: Prenatal assessment and outcome. Journal of Pediatric Surgery 39:319-323, 2004
  157. Flake AW. The conceptual application of systems theory to stem cell biology: A matter of context. Blood Cells, Molecules, and Diseases 32: 58-64, 2004
  158. Hayashi S, Hsieh MH, Peranteau WH, Ashizuka S, Flake AW. Complete allogeneic hematopoietic chimerism achieved by in utero hematopoietic cell transplantation and co-transplantation of LLME treated, MHC sensitized, donor lymphocytes. Experimental Hematology 32:290-299, 2004
  159. Javazon EH, Beggs KJ, Flake AW. Mesenchymal Stem Cells: Paradoxes of Passaging. Experimental Hematology 32:414-25, 2004
  160. Kirsch AJ, Macarak EJ, Chaqour B, Quinn T, Zderic S, Flake A, Crombleholme T, Adzick NS, Canning D, Hubbard AM, Rosenbloom J, Snyder HM, Howard PS. Molecular response of the bladder to obstruction. Adv Exp Med Biol. 2003;539(Pt A):195-216.
  161. Biard JM, Wilson RD, Johnson MP, Hedrick HL, Flake AW, Crombleholme TM, Adzick NS. Prenatally diagnosed giant omphaloceles: Short and long term outcomes. Prenatal Diagnosis 24:434-439, 2004
  162. Hedrick HL, Flake AW, Crombleholme TM, Howell LJ, Johnson MP, Wilson RD, Adzick NS. Sacrococcygeal teratoma: prenatal assessment, fetal intervention, and outcome. Journal of Pediatric Surgery 39:430-438, 2004
  163. Krupnick AS, Belsara KR, Kreisel D, Gelman A, Riha M, Sena-Esteves M, Rosengard BR, Flake AW. The fetal liver as a source of autologous progenitor cells for perinatal cardiac tissue engineering. Tissue Engineering 10:723-735, 2004
  164. Wilson RD, Baxter JK, Johnson MP, King M, Kasperski S, Crombleholme TM, Flake AW, Hedrick HL, Howell LJ, Adzick NS. Thoracoamniotic shunts: fetal treatment of pleural effusions and congenital cystic adenomatoid malformations. Fetal Diagnosis & Therapy 19:413-20, 2004.
  165. Krag TOB, Bogdanovich S, Jensen CJ, Fischer MD, Hansen-Schwarz J, Javazon EH, Flake AW, Edvinsson L, Khurana TS. Heregulin ameliorates the dystrophic phenotype in mdx mice. Proceedings of the National Academy of Science 101:13856-13860, 2004
  166. Tang XX, Robinson ME, Riceberg JS, Kim DY, Kung B, Titus TB, Hayashi S, Flake AW, Carpentieri D, Ikegaki N. Favorable neuroblastoma genes and molecular therapeutics of neuroblastoma. Clinical Cancer Research 10:5837-5844, 2004
  167. Rychik J, Tian Z, Cohen MS, Ewing SG, Cohen D, Howell LJ, Wilson RD, Johnson MP, Hedrick HL, Flake AW, Crombleholme TM, Adzick NS. Acute cardiovascular effects of fetal surgery in the human. Circulation. 110:1549-1556, 2004
  168. Midrio P, Faussone-Pellegrini MS, Vannucchi MG, Flake AW. Gastroschisis in the rat model is associated with a delayed maturation of intestinal pacemaker cells and smooth muscle cells. Journal of Pediatric Surgery. 39:1541-1547, 2004.
  169. Javazon EH, Merchant AM, Danzer E, Flake AW. Reconstitution of hematopoiesis following intrauterine transplantation of stem cells. Methods in Molecular Medicine 105:81-94, 2004.
  170. Wilson RD, Johnson MP, Flake AW, Crombleholme TM, Hedrick HL, Wilson J, Adzick NS. Reproductive outcomes following pregnancy complicated by maternal-fetal surgery. American Journal of Obstetrics and Gynecology 191:1430-1436, 2004
  171. MacKenzie TC, Kobinger GP, Louboutin J-P, Radu A, Shah N, Javazon E, Sena-Esteves M, Wilson JM, Flake AW. Transduction of Satellite Cells After Prenatal Intramuscular Administration of Lentiviral Vectors  Journal of Gene Medicine 7:50-58, 2005
  172. Danzer E, Johnson MP, Wilson RD, Flake AW, Hedrick HL, Sutton LN, Adzick NS. Fetal head biometry following in-utero repair of myelomeningocele. Ultrasound in Obstetrics and Gynecology. 24:606-611, 2004.
  173. Merchant AM, Flake AW. Surgeons and stem cells: a pragmatic perspective on shifting paradigms. Surgery 136:975-980, 2004
  174. Sena-Esteves M, Tebbets JC, Steffens S, Crombleholme T, Flake AW. Optimized large-scale production of high titer lentivirus vector pseudotypes. Journal of Virology Methods 122:131-9. 2004.
  175. Steffens S, Tebbets J, Cramm CM, Lindemann D, Flake A, Sena-Esteves M. Transduction of human glial and neuronal tumor cells with different lentivirus vector pseudotypes. Journal of Neuro-Oncology 70:281-288, 2004
  176. Keswani SG, Crombleholme TM, Pawel BR, Johnson MP, Flake AW, Hedrick HL, Howell LJ, Wilson RD, Davis GH, Adzick NS. Prenatal diagnosis and management of mainstem bronchial atresia. Fetal Diagnosis & Therapy 20:74-78, 2005
  177. Merchant AM, Hedrick HL, Crombleholme TM, Johnson MP, Wilson RD, Howell LJ, Adzick NS, Flake AW. Management of fetal mediastinal teratomas: A report of two cases.  Journal of Pediatric Surgery 40:228-231, 2005
  178. Baptista MJ, Melo-Rocha G, Pedrosa C, Gonzaga S, Teles A, Estevao-Costa J, Areias JC, Flake AW, Leite-Moreira AF, Correia-Pinto J. Antenatal vitamin A administration attenuates lung hypoplasia by interfering with early instead of late determinants of lung underdevelopment in congenital diaphragmatic hernia. Journal of Pediatric Surgery Apr;40(4):658-665, 2005. 
  179. Danzer E, Schwarz  U, Wehrli S, Radu A, Adzick NS, Flake AW. Retinoic acid induced myelomeningocele in fetal rats: characterization by histopathological analysis and magnetic resonance imaging. Experimental Neurology, 194:467-475, 2005
  180. Davey MG, Biard JM, Robinson L, Tsai J, Schwarz U, Danzer E, Adzick NS, Flake AW, Hedrick HL. Surfactant protein expression is increased in the ipsilateral but not contralateral lungs of fetal sheep with left-sided diaphragmatic hernia. Pediatric Pulmonology. 39(4):359-67. 2005
  181. Hedrick HL, Flake AW, Crombleholme TM, Howell LJ, Johnson MP, Wilson RD, Adzick. The ex utero intrapartum therapy procedure for high-risk fetal lung lesions. Journal of Pediatric Surgery. 40:1038-43, 2005.
  182. Keswani SG, Crombleholme TM, Rychik J, Tian Z, Mackenzie TC, Johnson MP, Wilson RD, Flake AW, Hedrick HL, Howell LJ, Adzick NS. Impact of continuous intraoperative monitoring on outcomes in open fetal surgery Fetal Diagnosis & Therapy 20:316-20 2005.
  183. Fogel MA, Wilson D, Flake A, Johnson M, Cohen D, McNeal G, Tian Z-Y, Rychick J. A new method of functional assessment of the fetal heart using a novel application of “real time” cardiac magnetic imaging. Fetal Diagnosis & Therapy, 20:475-480, 2005
  184. Wilson RD, Hedrick HL, Liechty KW, Flake AW, Johnson MP, Bebbington J, Adzick NS. Cystic adenomatoid malformation of the lung: Review of genetics, prenatal diagnosis, and in utero treatment. American Journal of Medical Genetics 140:151-155, 2006
  185. Liechty KW, Hedrick HL, Hubbard AM, Johnson MP, Wilson RD, Ruchelli ED, Howell LJ, Crombleholme TM, Flake AW, Adzick NS. Severe pulmonary hypoplasia associated with giant cervical teratomas. Journal of Pediatric Surgery 41:230-233, 2006.
  186. Ashizuka S, Peranteau WH, Hayashi S, Flake AW. Busulfan Conditioned Bone Marrow Transplantation Results In High Level Allogeneic Chimerism In Mice Made Tolerant By In Utero Hematopoietic Cell Transplantation. Experimental Hematology 34:359-368, 2006
  187. Danzer E, Robinson LE, Davey MG, Schwarz U, Volpe M, Adzick NS, Flake AW, Hedrick HL. Tracheal occlusion in fetal rats alters expression of mesenchymal nuclear transcription factors without effecting surfactant protein expression. Journal of Pediatric Surgery 41:774-780, 2006
  188. Haber BA,  Erlichman J, Thayu M, Flake AW, Rand EB. Successful Revision of Portoenterostomy in an Infant with Biliary Atresia. Journal of Pediatric Surgery 41(7):e1-e3, 2006
  189. Davey MG, Biard JM, Robinson L, Tsai J, Schwarz U, Danzer E, Adzick NS, Flake AW, Hedrick HL. Prenatal glucocorticoids and exogenous surfactant therapy improve lung compliance and gas exchange in lambs with diaphragmatic hernia undergoing fetal tracheal occlusion. Pediatric Research 60:131-135, 2006
  190. Danzer E, Hubbard AM, Hedrick HL, Johnson MP, Wilson RD, Howell LJ, Flake AW, Adzick NS. Diagnosis and characterization of fetal sacrococcygeal teratoma with prenatal imaging. American Journal of Roentgenology 187:W350-356 , 2006
  191. Shaaban AF, Kim HB, Liechty KW, Flake AW. Prenatal Transplantation of cytokine-stimulated marrow improves early chimerism in a resistant strain combination but results in poor long-term engraftment. Experimental Hematology 34:1277-1286, 2006
  192. Adibe OO, Nichol PF, Flake AW, Mattei P.Comparison of outcomes after laparoscopic and open pyloromyotomy at a high-volume pediatric teaching hospital.  Journal of Pediatric Surgery 41:1676-8. 2006
  193. Davey MG, Biard JM, Robinson L, Tsai J, Schwarz U, Danzer E, Adzick NS, Flake AW, Hedrick HL. Prenatal glucocorticoids improve lung morphology and partially restores surfactant mRNA expression in lambs with diaphragmatic hernia undergoing fetal tracheal occlusion. Pediatric Pulmonology. 41:1188-1196, 2006
  194. Peranteau WH, Endo M, Adibe O, Merchant A, Zoltick P, Flake AW. CD26 inhibition enhances allogeneic donor cell homing and engraftment after in utero hematopoietic cell transplantation. Blood 108:4268-4274, 2006
  195. Wilson RD, Pawel B, Bebbington M, Johnson MP, Lim F-Y, Stamilio D, Silber A, Zackai E, Flake AW.  Short Communication: Congenital pulmonary lymphangiectasis sequence: A rare, heterogeneous, and lethal etiology for prenatal pleural effusion. Prenatal Diagnosis 26:1058-1061, 2006.
  196. Peranteau WH, Endo M, Adibe O, Flake AW. Evidence for an adaptive immune barrier after in utero hematopoietic cell transplantation. Blood 109:1331-1333, 2007
  197. Danzer E, Kidoo DA, Redden RA, Robinson L, Radu A, Zderic SA, Doolin E, Adzick NS, Flake AW. Structural and functional characterization of bladder smooth muscle in fetal rats with retinoic acid induced myelomeningocele. American Journal of Physiology; Renal Physiology 292:F197-206, 2007
  198. Henriques-Coelho T,  Gonzaga S, Endo M, Zoltick PW, Davey MG, Leite-Moreira AF, Correia-Pinto J, Flake AW. Targeted gene transfer to the fetal rat lung interstitium by ultrasound guided intraparenchymal injection. Molecular Therapy 15:340-347,  2007
  199. Endo M, Zoltick PW, Chung DC, Bennett J, Flake AW. Gene transfer to ocular stem cells by early gestational intra-amniotic injection of lentiviral vector. Molecular Therapy 15:579-587, 2007.
  200. Badillo AT, Beggs K, Javazon EH, Tebbets J, Flake AW. Murine mesenchymal stem cells elicit an in vivo cellular and humoral alloimmune response. Biology of Blood and Bone Marrow Transplantation 13:412-422, 2007
  201. Javazon  EH, Keswani SG, Badillo AT, Crombleholme TM, Zoltick PW, Radu A, Kozin ED, Beggs K, Malik AA., Flake AW. Enhanced epithelial gap closure and increased angiogenesis in wounds of diabetic mice treated with adult murine mesenchymal stem cells. Wound Repair and Regeneration  15:350-359, 2007
  202. Wilson RD, Johnson MP, Bebbington M, Flake AW, Hedrick HL, Sutton LN, Adzick NS. Does a Myelomeningocele Sac Compared to No Sac Result in Decreased Postnatal Leg Function following Maternal Fetal Surgery for Spina Bifida Aperta? Fetal Diagnosis & Therapy 22:348-351, 2007.
  203. Netta DA, Wilson RD, Visintainer P, Johnson MP, Hedrick HL, Flake AW, Adzick NS. Gastroschisis: Growth Patterns and a Proposed Prenatal Surveillance Protocol. Fetalal Diagnosis & Therapy 22:352-357, 2007.
  204. Peranteau WH, Wilson RD, Liechty KW, Johnson MP, Bebbington MW, Hedrick HL, Flake AW, Adzick NS. Effect of Maternal Betamethasone Administration on Prenatal Congenital Cystic Adenomatoid Malformation Growth and Fetal Survival. Fetal Diagnosis & Therapy. 22:365-371, 2007.
  205. Merchant AM, Peranteau W, Wilson RD, Johnson MP, Bebbington MW, Hedrick HL, Flake AW, Adzick NS. Postnatal chest wall deformities after fetal thoracoamniotic shunting for congenital cystic adenomatoid malformation. Fetal Diagnosis & Therapy. 22:435-439, 2007
  206. Sabatino DE, MacKenzie TC,  Peranteau WH, Campagnoli C, Liu Y-L, Flake AW, High KA. Persistent expression of hF.IX after tolerance induction by in utero or neonatal administration of AAV-1-F.IX in hemophilia B mice. Molecular Therapy 15:1677-1685, 2007
  207. Hedrick HL, Danzer E, Merchant A, Bebbington MW, Zhao H, Flake AW, Johnson MP, Howell LJ, Wilson RD, Adzick NS. Liver position and lung-to-head ratio for prediction of extracorporeal membrane oxygenation and survival in isolated left congenital diaphragmatic hernia. American Journal of Obstetrics and Gynecology 197:422e1-4, 2007
  208. Davey M, Shegu S, Danzer E, Ruchelli E, Adzick NS, Flake AW, Hedrick HL. Pulmonary arteriole muscularization in lambs with diaphragmatic hernia after combined tracheal occlusion/glucocorticoids therapy. American Journal of Obstetrics and Gynecology 197:381e1-7, 2007.
  209. Bebbington MW, Danzer E, Hedrick HL, Flake AW, Wilson RD, Johnson MP, Adzick NS. OC223: Serial measurement of lung head ratio (LHR) does not predict survival in left-sided congenital diaphragmatic hernia (CDH). Ultrasound in Obstetrics and Gynecology 30:435, 2007
  210. Endo M, Zoltick PW, Peranteau WH, Radu A, Muvarak N, Ito M, Yang Z, Cotsarelis G, Flake AW. Efficient In Vivo Targeting of Epidermal Stem Cells by Early Gestational Intra-amniotic Injection of Lentiviral Vector Driven by the Keratin 5 Promoter. Molecular Therapy 16: 131-137, 2008 
  211. Tsai AY, Liechty KW, Hedrick HL, Bebbington M, Wilson RD, Johnson MP, Howell LJ, Flake AW, Adzick NS. Outcomes following postnatal resection of prenatally diagnosed asymptomatic cystic lung lesions. Journal of Pediatric Surgery 43:513-517, 2008
  212. Tiblad E, Wilson RD, Carr M, Flake AW, Hedrick HL, Johnson MP, Bebbington MW, Mann S, Adzick NS. OEIS sequence – a rare congenital anomaly with prenatal evaluation and postnatal outcome in six cases. Prenatal Diagnosis 28:141-147, 2008
  213. Badillo AT, Peranteau WH, Heaton TE, Quinn C, Flake AW. Murine Bone Marrow Derived Stromal Progenitor Cells Fail to Prevent or Treat Acute Graft Versus Host Disease. British Journal of Haematology 141:224-234, 2008
  214. Danzer E, Radu A, Robinson LE, Volpe MV, Adzick NS, Flake AW. Morphological analysis of the neuromuscular development of the anorectal unit in fetal rats with retinoic acid induced myelomeningocele Neuroscience Letters 430:157-162,  2008.
  215. Badillo AT, Hedrick HL, Wilson RD, Danzer E, Bebbington MW, Johnson MP, Liechty KL, Flake AW, Adzick NS. Prenatal ultrasonographic gastrointestinal abnormalities in fetuses with gastroschisis do not correlate with postnatal outcomes. Journal of Pediatric Surgery 43:647-653, 2008
  216. Biard JM, Schwarz U, Davey MG, Danzer E, Johnson MP, Flake AW, Adzick SN, Hedrick HL. Main bronchus occlusion for treatment of congenital diaphragmatic hernia in fetal lambs. Journal of Pediatric Surgery 43:620-626, 2008.
  217. Tran KM, Flake AW, Kalawadia NV, Maxwell LG, Rehman MA. Emergent excision of a prenatally diagnosed sacrococcygeal teratoma. Paediatric Anaesthesia. 18:431-434, 2008
  218. Laje P, Peranteau WH, Flake AW. Repair of Indirect Inguinal Hernias by Laparoscopic Joining of the Edges. Surgical Laparoscopic and Endoscopic Percutaneous Techniques.18:280-282, 2008
  219. Ibele A, Flake AW, Shaaban A. Survival of a profoundly hydropic fetus with a sacrococcygeal teratoma delivered at 27 weeks of gestation for maternal mirror syndrome. Journal of Pediatric Surgery. 43:e17-20, 2008
  220. Behrens EM, Ning Y, Muvarak N, Zoltick PW, Flake AW, Gallucci S, Apoptotic Cell-mediated immunoregulation of dendritic cells does not require iC3b opsonization. Journal of Immunology, 181:3018-3026, 2008
  221. Peranteau WH, Merchant AM, Hedrick HL, Liechty KW, Howell LJ, Flake AW, Wilson RD, Johnson MP, Bebbington MW, Adzick NS.  Prenatal Course and Postnatal Management of Peripheral Bronchial Atresia: Association with Congenital Cystic Adenomatoid Malformation of the Lung. Fetal Diagnosis & Therapy. 24:190-196. 2008
  222. Mong A, Johnson AM, Kramer SS, Coleman BG, Hedrick HL, Kreiger P, Flake A, Johnson M, Wilson RD, Adzick NS, Jaramillo D. Congenital High Airway Obstruction Syndrome: MR/US findings, effect on management, and outcome. Pediatric Radiology, 38:1171-1179, 2008
  223. Gonzaga S, Henriques-Coelho T, Davey M, Zoltick PW, Leite-Moreira AF, Correia-Pinto J, Flake AW. Cystic Adenomatoid Malformations are Induced by Localized FGF10 overexpression in the fetal rat lung. American Journal of Respiratory Cell and Molecular Biology. 39:346-355 2008.
  224. Danzer E, Davey MG, Kreiger PA, Ruchelli ED, Johnson MP, Adzick NS, Flake AW, Holly L Hedrick. Fetal tracheal occlusion for severe congenital diaphragmatic hernia in humans: A morphometric study of lung parenchyma and muscularization of Pulmonary Arterioles. Journal of Pediatric Surgery, 43:1767-1775, 2008
  225. Danzer E, Adzick NS, Rintoul NE, Zarnow DM, Schwartz ES, Melchionni J, Flake AW, Sutton LN, Johnson MP. Intradural inclusion cysts following in utero myelomeningocele closure: clinical implications and follow-up. Journal of Neurosurgery Pediatrics, 2:406-413, 2008
  226. Wilson RD, Hedrick H, Flake AW, Johnson MP, Bebbington MW, Mann S, Rychik J, Liechty K, Adzick NS. Sacrococcygeal Teratomas: Prenatal Surveillance, Growth and Pregnancy Outcome. Fetal Diagnosis & Therapy, 25:15-20. 2008
  227. Danzer E, Ernst LM, Rintoul NE, Johnson MP, Adzick NS, Flake AW. In utero meconium passage in fetuses and newborns with myelomeningocele. Journal of Neurosurgery Pediatrics 3:141-146, 2009
  228. Niiya M, Endo M, Zoltick PW, Muvarak NE, Motto DG, Ginsburg D, Flake AW, Zheng XL. Correction of Adamts13-deficiency by in utero gene transfer of lentiviral vector encoding ADAMTS13 genes. Molecular Therapy 17:34-41, 2009
  229. Deprest JA, Hyett JA, Flake AW, Nicolaides K, Gratacos E. Current controversies in prenatal diagnosis 4: Should fetal surgery be done in all cases of severe diaphragmatic hernia? Prenat Diagn. 29:15-19, 2009.
  230. Peranteau WH, Heaton TE, Gu Y-C, Volk SW, Bauer TR, Alcorn K, Tuschong LM, Johnson MP, Hickstein DD, Flake AW. Haploidentical In Utero Hematopoietic Cell Transplantation Improves Phenotype and Can Induce Tolerance for Postnatal Same Donor Transplants in the Canine Leukocyte Adhesion Deficiency Model.  Biology of Blood and Bone Marrow Transplantation 15:293-305, 2009
  231. Laje P, Shang D, Cao W, Niija M, Endo M, Radu A, DeRogatis N, Scheiflinger F, Zoltick PW, Flake AW, Zheng XL, Correction of ADAMTS13 deficiency by hematopoietic progenitor cell-mediated gene therapy. Blood 113:2172-2180, 2009
  232. Merianos DJ, Tiblad E, Santore MT, Laje P, Endo M, Zoltick PW, Flake AW. Maternal alloantibodies transferred via breast milk induce a postnatal immune response that breaks tolerance after in utero hematopoietic cell transplantation. Journal of Clinical Investigation 119:2590-2600, 2009
  233. Nallasamy S, Davidson SL, Howell LJ, Hedrick H, Flake AW, Crombleholme TM, Adzick NS, Young TL. The effects of fetal surgery on retinopathy of prematurity development. Opthalmology and Eye Diseases 1:13-19, 2009
  234. Endo M, Henriques-Coelho T, Zoltick PW, Stitelman DH, Peranteau WH, Radu A, Flake AW. Developmental stage determines distribution and duration of gene expression after early intra-amniotic gene transfer using lentiviral vectors. Gene Therapy 17:61-71, 2010.
  235. Laje P, Muvarak N, Zoltick PW, Flake AW. A comparison of transduction efficiency and long-term repopulating capacity of lentiviral transduced SLAM versus KSL-enriched HSC using an abbreviated transduction protocol. Gene Therapy 17:412-418, 2010
  236. Danzer E, Gerdes M, D’Agostino J, Bernbaum J, Siegle J, Hoffman C, Rintoul NE, Liechty KW, Adzick NS, Flake AW, Hedrick HL: Prospective, interdisciplinary follow-up of children with prenatally diagnosed giant omphalocele: short-term neurodevelopmental outcome. Journal of Pediatric Surgery 45:718-723, 2010.
  237. Watanabe M, Jo J, Radu A, Kaneko M, Tabata Y, Flake AW. A tissue engineering approach for prenatal closure of myelomeningocele with gelatin sponges incorporating basic fibroblast growth factor. Tissue Engineering Part A 16:1645-1655, 2010
  238. Danzer E, Layne MD, Auber F, Shegu S, Kreiger P, Radu A, Volpe M, Adzick NS, Flake AW. Gastroschisis in mice lacking aortic carboxypeptidase-like protein (ACLP) is associated with a defect in neuromuscular development restricted to the eviscerated intestine: early characterization and histomorphological assessment.  Pediatric Research 68:23-28, 2010
  239. Laje P, Flake AW, Adzick NS Prenatal diagnosis and postnatal resection of intraabdominal enteric duplications. Journal of Pediatric Surgery 45:1554-1558, 2010
  240. Laje P, Clark FH, Friedman JR, Flake AW.  Increased susceptibility to liver damage from pneumoperitoneum in a murine model of biliary atresia.  Journal of Pediatric Surgery 45:1554-1558, 2010
  241. Roybal J, Liechty KW, Hedrick HL, Bebbington M, Johnson MP, Coleman B, Adzick NS, Flake AW. Predicting severity of Congenital High Airway Obstruction Syndrome. Journal of Pediatric Surgery 45:1633-1639, 2010
  242. Danzer E, Gerdes M, Bernbaum J, D'Agostino J, Bebbington MW, Siegle J, Hoffman C, Rintoul NE, Flake AW, Adzick NS, Hedrick HL. Neurodevelopmental outcome of infants with congenital diaphragmatic hernia prospectively enrolled in an interdisciplinary follow-up program. Journal of Pediatric Surgery. 45:1759-1766, 2010.
  243. Kaye AJ, Rand EB, Munoz P, Spinner NB, Flake AW, Kamath BM. Effect of Kasai Procedure on the Hepatic Outcome in Alagille Syndrome. Journal of Pediatric Gastroenterology and Nutrition 51:319-321, 2010.
  244. Bertozzi CC, Schmaier AA, Mericko P, Hess P, Zou Z, Chen M, Chen C-Y, Xu B, Lu MM, Zhou D, Sebzda E, Santore MT, Meriano DJ, Stadfeld M, Flake AW, Graf T, Skoda R, Maltzman JS, Koretzky GA, Kahn ML. Platelets regulate lymphatic vascular development through CLEC-2-SLP-76 signalling. Blood 116:661-670, 2010
  245. Stitelman DH, Endo M, Bora A, Muvarak N, Zoltick PW, Flake AW, Brazelton TR. Robust in vivo transduction of nervous system and neural stem cells by early gestational intra-amniotic gene transfer using lentiviral vector. Molecular Therapy, 18:1615-1623, 2010
  246. Wilson RD, Lemerand K, Johnson MP, Flake AW, Bebbington M, Hedrick HL, Adzick NS. Reproductive outcomes in subsequent pregnancies after a pregnancy complicated by open maternal-fetal surgery (1996-2007). American Journal of Obstetrics and Gynecology. 209 e1-6, 2010
  247. Santore MT, Behar BJ, Blinman TA,  Doolin EJ, Hedrick HL, Mattei P, Nance ML, Adzick NS, Flake AW. Hepaticoduodenostomy versus hepaticojejunostomy for reconstruction after resection of choledochal cyst. Journal of Pediatric Surgery 46:209-213, 2011
  248. Danzer E, Zhang L, Radu A, Bebbington MW, Liechty KW, Adzick NS, Flake AW. Amniotic fluid levels of glial fibrillary acidic protein in fetal rats with retinoic acid induced myelomeningocele: a potential marker for spinal cord injury. American Journal of Obstetrics and Gynecology 204:178.e1-178.e11, 2011
  249. Roybal JL, Moldenhauer JS, Khalek N, Bebbington MW, Johnson MP, Hedrick HL, Adzick NS, Flake AW. Early delivery as an alternative management strategy for select high –risk fetal sacrococcygeal teratomas. Journal of Pediatric Surgery 46:1325-1332, 2011
  250. Watanabe, M, Li H, Roybal JL, Santore MT, Radu A, Jo J, Kaneko M, Tabata Y, Flake AW. A Tissue Engineering Approach for Prenatal Closure of Myelomeningocele: Comparison Of Gelatin Sponge And Microsphere Scaffolds And Bioactive Protein Coatings. Tissue Engineering (7-8):1099-110, 2011
  251. Adzick NS, Thom EA, Spong CY, Brock JW 3rd, Burrows PK, Johnson MP, Howell LJ, Farrell JA, Dabrowiak ME, Sutton LN, Gupta N, Tulipan NB, D'Alton ME, Farmer DL; MOMS Investigators.  A randomized trial of prenatal versus postnatal repair of myelomeningocele. New England Journal of Medicine. 364:993-1004, 2011.
  252. Roybal JL, Endo M, Radu A, Zoltick PW, Flake AW. Early gestational gene transfer of IL-10 by systemic administration of lentiviral vector can prevent arthritis in a murine model. Gene Therapy 18:719-726, 2011.
  253. Hakguder G, Olguner M, Gurel D, Akgur FM, Flake AW. Induction of fetal diuresis with intraamniotic furosemide injection reduces intestinal damage in a rat model of gastroschisis. European Journal of Pediatric Surgery 21:183-18, 2011
  254. McCloskey JJ, Motas D, Cohen D, Jarcher MD, Flake AW. Fluoroscopic-assisted endobronchial intubation for single lung ventilation in infants. Pediatric Anesthesia 6:681-684, 2011
  255. Santore MT, Deans KJ, Behar BJ, Blinman TA, Adzick NS, Flake AW. Laparoscopic hepaticoduodenostomy versus open hepaticoduodenostomy for reconstruction after resection of choledochal cyst. Journal of Laparoendoscopic & Advanced Surgical Techniques. 21:375-378, 2011
  256. Davey MG, Zoltick PW, Todorow CA, Limberis MP, Hedrick HL, Flake AW. Jaagsiekte sheep retrovirus pseudotyped lentivirus-mediated gene transfer to fetal ovine lung. Gene Therapy 19:201-209, 2012
  257. Superina R, Magee JC, Brandt ML, Healy PJ, Tiao G, Ryckman F, Karrer FM, Iyer K, Fecteau A, West K, Burns RC, Flake A, Lee H, Dillon P, Colombani P, Ricketts R, Li Y, Moore J, Wang KS; The Childhood Liver Disease Research and Education Network. The anatomic pattern of biliary atresia identified at the time of Kasai Hepatoportoenterostomy and early postoperative clearance of jaundice are significant predictors of transplant free survival. Annals of Surgery 254:577-585, 2011.
  258. Endo M, Zoltick PW, Radu A, Qiujie J, Matsui C, Marinkovich PM, McGrath J, Tamai K, Uitto J, Flake AW. Early Intra-amniotic gene transfer using lentiviral vector improves skin phenotype in a murine model of Herlitz junctional epidermolysis bullosa. Gene Therapy 19:561-569, 2012
  259. Danzer E, Victoria T, Bebbington MW, Siegle J, Rintoul NE, Johnson MP, Flake AW, Adzick NS, Hedrick HL. Fetal MRI-calculated total lung volumes in the prediction of short term outcome in giant omphalocele – Preliminary findings. Fetal Diagnosis and Therapy 31:248-253, 2012
  260. Danzer E, Zarnow D, Gerdes M, D’Augustino JA, Siegle J, Bebbington MW, Flake AW, Adzick NA, Hedrick HL. Abnormal brain development and maturation on MR imaging in survivors of severe congenital diaphragmatic hernia. Journal of Pediatric Surgery 47:453-461, 2012
  261. Roybal JL, Endo M, Radu A, Gray L, Todorow CA, Zoltick PW, Lutsenko S, Flake AW. Early gestational gene transfer with lentiviral vector driven by a liver promotor targets the fetal liver and improves phenotype in a murine model of Wilson’s Disease. Gene Therapy 19:1085-1094, 2012
  262. Tsai J, Sulkowski J, Adzick NS, Hedrick HL, Flake AW. Patch repair for congenital diaphragmatic hernia: Is it really a problem? Journal of Pediatric Surgery, 47:637-641, 2012.
  263. Danzer E, Siegle J., D’Agostino J, Gerdes M, Hoffman C, Bernbaum J, Rintoul NE, Flake AW, Adzick NS, Hedrick HL. Early neurodevelopmental outcome of infants with high-risk fetal lung lesions. Fetal Diagnosis and Therapy 31:210-215, 2012
  264. Buchlis G, Podsakoff GM, Radu A, Hawk SM, Flake AW, Mingozzi F, High KA. Factor IX expression in skeletal muscle of a severe hemophilia B patient 10 years after AAV-mediated gene transfer. Blood 119:3038-3041, 2012
  265. Victoria T, Bebbington MW, Danzer E, Flake AW, Johnson MP, Dinan D, Adzick NS, , Hedrick HL. Use of Magnetic Resonance Imaging in Prenatal Prognosis of the Fetus with Isolated Left Congenital Diaphragmatic Hernia. Prenatal Diagnosis 32:715-723,  2012
  266. Laje P, Johnson MP, Howell LJ, Bebbington MW, Hedrick HL, Flake AW, Adzick NS. Ex utero intrapartum treatment in the management of giant cervical teratomas. Journal of Pediatric Surgery  47:1208-1216, 2012
  267. Osterhoudt KC, Peranteau WH, Shaw KN, Flake AW. 2 year-old girl with abdominal pain after an action sure to attract attention. Pediatric Emergency Care 28:1406-1408, 2012
  268. Wu C, Endo M, Yang BH, Radecki MA, Spivak RM, Davis PF, Zoltick PW, Flake AW, Kirschner RA, Nah H-D. Intra-amiotic transient transduction of the periderm with a viral vector encoding TGFb3 prevents cleft palate in Tgfb3(-/-) embryos. Molecular Therapy 21:8-17, 2013
  269. Mueli M, Moehrlen U, Flake A, Ochsenbein N, Huesler M, Biro P, Sheer I, Tharakan S, Durig P, Zimmerman R. Fetal surgery in Zurich: Key features of our first open in utero repair of myelomeningocele. European Journal of Pediatric Surgery 23:494-498, 2013.
  270. Danzer E, Gerdes M, D’Agostino J, Partridge E, Hoffman-Craves C, Burnbaum J, Rintoul NE, Flake AW, Adzick NS, Hedrick HL: Preschool neurological assessment in congenital diaphragmatic hernia survivors: Outcome and perinatal factors associated with neurodevelopmental impairment. Early Human Development 89:393-400, 2013
  271. Danzer E, Gerdes M, D’Agostino J,  Hoffman C, Burnbaum J, Bebbington MW, Siegle J, Sulkowski J, Rintoul NE, Flake AW, Adzick NS, Hedrick HL. Longitudinal neurodevelopmental and neuromotor outcome in congenital diaphragmatic hernia patients in the first 3 years of life. Journal of Perinatology 33:893-898, 2013
  272. Meuli M, Meuli-Simmen C, Flake AW, Zimmerman R, Ochsenbein N, Scheer I, Mazzone L, Mohrlen U. Premiere use of integra artificial skin to close an extensive fetal skin defect during open in utero repair of myelomeningocele. Pediatric Surgical International 29:1321-1326, 2013.
  273. Laje P, Howell LJ, Johnson MP, Hedrick HL, Flake AW, Adzick NS. Perinatal management of congenital oropharyngeal tumors: The ex utero intrapartum treatment (EXIT) approach. Journal of Pediatric Surgery 48:2005-2010, 2013.
  274. Sundaram SS, Alonso EM, Haber B, Magee JC, Fredericks E, Kamath B, Kerkar N, Rosenthal P Shepherd R, Limbers C, Varni JW, Robuck P, Sokol RJ; Childhood Liver Disease Research and Education Network (ChiLDREN). Health related quality of life in patients with biliary atresia surviving with their native liver. Journal of Pediatrics 163:1052-1057, 2013
  275. Partridge EA, Canning DA, Long C, Peranteau WH, Hedrick HL, Adzick NS, Flake AW. Urologic and anorectal complications of sacrococcygeal teratomas: prenatal and postnatal predictors. Journal of Pediatric Surgery 49:139-43, 2014
  276. Peranteau WH, Moldenhauer JS, Khalek N, Martinez-Poyer JL, Howell LJ, Johnson MP, Flake AW, Adzick NS, Hedrick HL. Open fetal surgery for central bronchial atresia. Fetal Diagnosis and Therapy, (In Press) 2014
  277. Partridge EA, Bridge C, Donaher JG, Herkert L, Grill E, Danzer E, Gerdes M, Hoffman CH, D’Agostino J, Bernbaum JC, Rintoul NE, Peranteau W, Flake AW, Adzick NS, Hedrick HL. Incidence and factors associated with sensorineural and conductive hearing loss among survivors of congenital diaphragmatic hernia. Journal of Pediatric Surgery 49:890-894, 2014
  278. Tsai J, Blinman TA, Collins JL, Laje P, Hedrick HL, Adzick NS, Flake AW. The contribution of hiatal hernia to severe gastroesophageal reflux disease in patients with gastroschisis. Journal of Pediatric Surgery 49:395-398, 2014
  279. Joyeux L, Danzer E, Limberis M, Zoltick PW, Radu A, Flake AW, Davey MG. In utero lung gene transfer using AAV and lentiviral vectors in mice.  Human Gene Therapy Methods (e-pub ahead of press), 2014.
  280. Bezerra JA, Spino C, Magee JC, Shneider BL, Rosenthal P, Wang KS, Erlichman J, Haber B, Hertel PM, Karpen SJ, Kerkar N, Loomes KM, Molleston JP, Murray KF, Romero R, Schwarz KB, Shepherd R, Suchy FJ, Turmelle YP, Whitington PF, Moore J, Sherker AH, Robuck PR, Sokol RJ; Childhood Liver Disease Research and Education Network (ChiLDREN). Use of corticosteroids after hepatoportoenterostomy for bile drainage in infants with biliary atresia: the START randomized clinical trial.  JAMA. 311:1750-9. 2014.
  281. Vrecenak JD, Pearson EG, Santore MT, Todorow CA, Li H, Radu A, Johnson MP, Flake AW. Stable long-term mixed hematopoietic chimerism achieved in a canine model of allogeneic in utero hematopoietic cell transplantation. Blood (e-pub ahead of press), 2014
  282. Stitelman DH, Brazelton TR, Bora A, Traas J, Joyeux L, Merianos D, Davey MA, Flake AW. Developmental stage determines the efficacy of gene transfer to muscle satellite cells by in utero delivery of Adeno-Associated Virus Vector. Molecular Therapy – Methods and Clinical Development, (in Press) 2014
  283. Rychik J, Cohen D, Tran KM, Szwast A, Natarajan SS, Johnson MP, Moldenhauer JS, Khalek N, Martinez-Poyer J, Flake AW, Hedrick HL, Adzick NS. The role of echocardiography in the operative management of the fetus undergoing myelomeningocele repair. Fetal Diagnosis and Therapy (Epub ahead of print) 2014
  284. Partridge EM, Victoria T, Coleman BG, Martinez-Poyer J, Laje P, Hedrick HL, Flake AW, Adzick NS. Prenatal diagnosis of esophageal bronchus – First report of a rare foregut malformation. Journal of Pediatric Surgery (Accepted) 2014
 
Awards, Honors and Membership in Honorary Societies
2008 - 2011 Listed in the Consumer Research Councils Guide to America’s Top Surgeons
 
2007 Dr. Rodman L. Sheen and Thomas G. Sheen Award of the American College of Surgeons
 
2006 Elected Cincinnati Children’s Hospital Medical Center Alumni “Hall of Honor”
 
2001 - 2011 Philadelphia Magazine “Best Docs for Kids”
 
1999 Invited “Meet the Expert”: American Society of Hematology
 
1998 - 2011 Listed in “Best Doctors in America”
 
1995 Michigans “Best Doctors”
 
1994 Councilman Association for Academic Surgery
 
1994 UCSF Residents Recognition for Excellence in Teaching Award
 
1991 - 1993 American College of Surgeons Fellowship
 
1985 Young Investigators Award, American Academy of Pediatrics, Surgical Section
 
1980 Alpha Omega Alpha
 
1980 James Gentry Thomas Memorial Award
 
1980 Edward Forrest Ellis Award
 
1979  Barton Scholarship
 
1978  Barton Scholarship
 
1979 Arnold Schlumberger Award for Excellence in Pathology
 
1978 Buchanon Key
 
1978  Phi Beta Kappa
 
1977 Univ. of Arkansas Honors Day Award in Chemistry
 
1976 - 1977  Coulter Jones Scholarship
 
1974 - 1977 Univ. of Arkansas Chemistry Scholar Award
 
1976 - 1976  National Science Foundation Research Scholarship
 
1976 Merck Index Award

Dr. Alan Flake

 
footer Terms of Use Legal Disclaimer Site Map Contact Us Financial Assistance About Penn Medicine Privacy Statement
penn medicine